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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanothermobacter marburgensis Methyl-coenzyme M reductase I subunit alpha (mcrA), partial CSB-YP320882MSQ
CSB-EP320882MSQ
CSB-BP320882MSQ
CSB-MP320882MSQ
CSB-EP320882MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Methyl-coenzyme M reductase I operon protein C (mcrC) CSB-YP320883MSQ
CSB-EP320883MSQ
CSB-BP320883MSQ
CSB-MP320883MSQ
CSB-EP320883MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidaminococcus fermentans (R)-2-hydroxyglutaryl-CoA dehydratase subunit alpha CSB-YP320884AWA
CSB-EP320884AWA
CSB-BP320884AWA
CSB-MP320884AWA
CSB-EP320884AWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Major urinary protein 4 (Mup4) CSB-YP320886MO
CSB-EP320886MO
CSB-BP320886MO
CSB-MP320886MO
CSB-EP320886MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP320887ILK
CSB-EP320887ILK
CSB-BP320887ILK
CSB-MP320887ILK
CSB-EP320887ILK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Protein P1-P2 (ORF1/ORF2), partial CSB-YP320888PQV1
CSB-EP320888PQV1
CSB-BP320888PQV1
CSB-MP320888PQV1
CSB-EP320888PQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Minor capsid protein P3-RTD (ORF3/ORF5), partial CSB-YP320889PQV
CSB-EP320889PQV
CSB-BP320889PQV
CSB-MP320889PQV
CSB-EP320889PQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6B (NHP6B) CSB-YP320890SVG
CSB-EP320890SVG
CSB-BP320890SVG
CSB-MP320890SVG
CSB-EP320890SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Quinate repressor protein (qa-1s), partial CSB-YP320891NHA
CSB-EP320891NHA
CSB-BP320891NHA
CSB-MP320891NHA
CSB-EP320891NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petunia hybrida Chalcone--flavonone isomerase A (CHI1) CSB-YP320892EUD
CSB-EP320892EUD
CSB-BP320892EUD
CSB-MP320892EUD
CSB-EP320892EUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide-exchange factor SEC12 (SEC12), partial CSB-YP320893SVG
CSB-EP320893SVG
CSB-BP320893SVG
CSB-MP320893SVG
CSB-EP320893SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4) CSB-YP320894TGA
CSB-EP320894TGA
CSB-BP320894TGA
CSB-MP320894TGA
CSB-EP320894TGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antibacterial protein 1 CSB-YP320895SLF
CSB-EP320895SLF
CSB-BP320895SLF
CSB-MP320895SLF
CSB-EP320895SLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP320896FKI
CSB-EP320896FKI
CSB-BP320896FKI
CSB-MP320896FKI
CSB-EP320896FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nuclear fusion protein BIK1 (BIK1) CSB-YP320897SVG
CSB-EP320897SVG
CSB-BP320897SVG
CSB-MP320897SVG
CSB-EP320897SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable proton ATPase 1A (H1A), partial CSB-YP320898LOE
CSB-EP320898LOE
CSB-BP320898LOE
CSB-MP320898LOE
CSB-EP320898LOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas punctata Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP320899AXY
CSB-EP320899AXY
CSB-BP320899AXY
CSB-MP320899AXY
CSB-EP320899AXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 32 CSB-YP320900LHY
CSB-EP320900LHY
CSB-BP320900LHY
CSB-MP320900LHY
CSB-EP320900LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa GDP-mannose 6-dehydrogenase (algD) CSB-YP320901EZX
CSB-EP320901EZX
CSB-BP320901EZX
CSB-MP320901EZX
CSB-EP320901EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabinose operon regulatory protein (araC) CSB-YP320902DTO
CSB-EP320902DTO
CSB-BP320902DTO
CSB-MP320902DTO
CSB-EP320902DTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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