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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Serine proteases 1/2 (Jon99Cii) CSB-YP321509DLU
CSB-EP321509DLU
CSB-BP321509DLU
CSB-MP321509DLU
CSB-EP321509DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Leu/Ile/Val/Thr-binding protein (livJ) CSB-YP321510SXB
CSB-EP321510SXB
CSB-BP321510SXB
CSB-MP321510SXB
CSB-EP321510SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sex-determining protein fem-1 (fem-1), partial CSB-YP321511CXY
CSB-EP321511CXY
CSB-BP321511CXY
CSB-MP321511CXY
CSB-EP321511CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I reaction center subunit VIII (psaI), partial CSB-YP321512FKI
CSB-EP321512FKI
CSB-BP321512FKI
CSB-MP321512FKI
CSB-EP321512FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Mercuric reductase (merA), partial CSB-YP321513TOA
CSB-EP321513TOA
CSB-BP321513TOA
CSB-MP321513TOA
CSB-EP321513TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major ferric iron-binding protein (fbp) CSB-YP321514NFZ
CSB-EP321514NFZ
CSB-BP321514NFZ
CSB-MP321514NFZ
CSB-EP321514NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila hydei Histone H1 (His1) CSB-YP321515DLM
CSB-EP321515DLM
CSB-BP321515DLM
CSB-MP321515DLM
CSB-EP321515DLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Envelope glycoprotein gp160 (env), partial CSB-YP321516SGW
CSB-EP321516SGW
CSB-BP321516SGW
CSB-MP321516SGW
CSB-EP321516SGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 30S ribosomal protein S7 (rpsG) CSB-YP321517TNT
CSB-EP321517TNT
CSB-BP321517TNT
CSB-MP321517TNT
CSB-EP321517TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Colicin I receptor (cirA) CSB-YP321518ENV
CSB-EP321518ENV
CSB-BP321518ENV
CSB-MP321518ENV
CSB-EP321518ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glyceraldehyde-3-phosphate dehydrogenase 3 (gpd-3) CSB-YP321519CXY
CSB-EP321519CXY
CSB-BP321519CXY
CSB-MP321519CXY
CSB-EP321519CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morganella morganii Urease subunit beta (ureB) CSB-YP321520MII
CSB-EP321520MII
CSB-BP321520MII
CSB-MP321520MII
CSB-EP321520MII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanine nucleotide-binding protein subunit beta-1 (gpb-1) CSB-YP321521CXY
CSB-EP321521CXY
CSB-BP321521CXY
CSB-MP321521CXY
CSB-EP321521CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Major core protein 4b (FPV167), partial CSB-YP321522FEY
CSB-EP321522FEY
CSB-BP321522FEY
CSB-MP321522FEY
CSB-EP321522FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein N1 (N1L) CSB-YP321523VAI
CSB-EP321523VAI
CSB-BP321523VAI
CSB-MP321523VAI
CSB-EP321523VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP321524HND
CSB-EP321524HND
CSB-BP321524HND
CSB-MP321524HND
CSB-EP321524HND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human papillomavirus type 31 Major capsid protein L1 (L1), partial CSB-YP321525HND
CSB-EP321525HND
CSB-BP321525HND
CSB-MP321525HND
CSB-EP321525HND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype B/C subtype adw Large envelope protein (S), partial CSB-YP321526HDS1
CSB-EP321526HDS1
CSB-BP321526HDS1
CSB-MP321526HDS1
CSB-EP321526HDS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate reductase flavoprotein subunit (frdA), partial CSB-YP321527WBQ
CSB-EP321527WBQ
CSB-BP321527WBQ
CSB-MP321527WBQ
CSB-EP321527WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Skin secretory protein xP2 (p2) CSB-YP321528XBE
CSB-EP321528XBE
CSB-BP321528XBE
CSB-MP321528XBE
CSB-EP321528XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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