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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli HTH-type transcriptional regulator BetI (betI) CSB-YP321529ENV
CSB-EP321529ENV
CSB-BP321529ENV
CSB-MP321529ENV
CSB-EP321529ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cymbidium ringspot virus RNA silencing suppressor p19 (ORF4) CSB-YP321530CVN
CSB-EP321530CVN
CSB-BP321530CVN
CSB-MP321530CVN
CSB-EP321530CVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine herpesvirus 1.1 Envelope glycoprotein B (gB), partial CSB-YP321531BJU
CSB-EP321531BJU
CSB-BP321531BJU
CSB-MP321531BJU
CSB-EP321531BJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP321532SYN
CSB-EP321532SYN
CSB-BP321532SYN
CSB-MP321532SYN
CSB-EP321532SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homeobox protein ceh-12 (ceh-12) CSB-YP321533CXY
CSB-EP321533CXY
CSB-BP321533CXY
CSB-MP321533CXY
CSB-EP321533CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis arietans Disintegrin bitan CSB-YP321534BTG
CSB-EP321534BTG
CSB-BP321534BTG
CSB-MP321534BTG
CSB-EP321534BTG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Minor capsid protein P3-RTD (ORF3/ORF5), partial CSB-YP321535PQW
CSB-EP321535PQW
CSB-BP321535PQW
CSB-MP321535PQW
CSB-EP321535PQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD) CSB-YP321536PVN
CSB-EP321536PVN
CSB-BP321536PVN
CSB-MP321536PVN
CSB-EP321536PVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Alanine dehydrogenase (ald) CSB-YP321537GFM
CSB-EP321537GFM
CSB-BP321537GFM
CSB-MP321537GFM
CSB-EP321537GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cyanate transport protein CynX (cynX), partial CSB-YP321538ENV
CSB-EP321538ENV
CSB-BP321538ENV
CSB-MP321538ENV
CSB-EP321538ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Latency-related protein 2 CSB-YP321539HWZ
CSB-EP321539HWZ
CSB-BP321539HWZ
CSB-MP321539HWZ
CSB-EP321539HWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe GTP-binding protein ypt3 (ypt3) CSB-YP321541SXV
CSB-EP321541SXV
CSB-BP321541SXV
CSB-MP321541SXV
CSB-EP321541SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP321542BRJ
CSB-EP321542BRJ
CSB-BP321542BRJ
CSB-MP321542BRJ
CSB-EP321542BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Chaperone protein DnaJ (dnaJ) CSB-YP321543BRJ
CSB-EP321543BRJ
CSB-BP321543BRJ
CSB-MP321543BRJ
CSB-EP321543BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PRD1 Protein P19 (XIX) CSB-YP321544EDO
CSB-EP321544EDO
CSB-BP321544EDO
CSB-MP321544EDO
CSB-EP321544EDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Insulin-like growth factor I (IGF1) CSB-YP321545GU
CSB-EP321545GU
CSB-BP321545GU
CSB-MP321545GU
CSB-EP321545GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Fe] (sodB) CSB-YP321546MVZ
CSB-EP321546MVZ
CSB-BP321546MVZ
CSB-MP321546MVZ
CSB-EP321546MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aplysia parvula ELH type 1 (ELH1) CSB-YP321547AMP
CSB-EP321547AMP
CSB-BP321547AMP
CSB-MP321547AMP
CSB-EP321547AMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A RNA-directed RNA polymerase, partial CSB-YP321548RGW
CSB-EP321548RGW
CSB-BP321548RGW
CSB-MP321548RGW
CSB-EP321548RGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer Mu-ctenitoxin-Pn1a CSB-YP321549EUV
CSB-EP321549EUV
CSB-BP321549EUV
CSB-MP321549EUV
CSB-EP321549EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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