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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Agrobacterium tumefaciens Wide host range virA protein (virA), partial CSB-YP321611AYS
CSB-EP321611AYS
CSB-BP321611AYS
CSB-MP321611AYS
CSB-EP321611AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine parvovirus Initiator protein NS1 (NS1), partial CSB-YP321612PQB
CSB-BP321612PQB
CSB-MP321612PQB
CSB-EP321612PQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope protein US9 homolog (76) CSB-YP321613EFI
CSB-EP321613EFI
CSB-BP321613EFI
CSB-MP321613EFI
CSB-EP321613EFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uracil phosphoribosyltransferase (FUR1) CSB-YP321614SVG
CSB-EP321614SVG
CSB-BP321614SVG
CSB-MP321614SVG
CSB-EP321614SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Larvicidal toxin 51 kDa protein (binB) CSB-YP321615LRU
CSB-EP321615LRU
CSB-BP321615LRU
CSB-MP321615LRU
CSB-EP321615LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Probable periplasmic serine endoprotease DegP-like (htrA) CSB-YP321616DSB
CSB-EP321616DSB
CSB-BP321616DSB
CSB-MP321616DSB
CSB-EP321616DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia sp. Actin, clone 302 CSB-YP321617AOF
CSB-EP321617AOF
CSB-BP321617AOF
CSB-MP321617AOF
CSB-EP321617AOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Transactivator/viroplasmin protein (ORF VI), partial CSB-YP321619CFE
CSB-EP321619CFE
CSB-BP321619CFE
CSB-MP321619CFE
CSB-EP321619CFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomycin 6-kinase (sph) CSB-YP321620SOG
CSB-EP321620SOG
CSB-BP321620SOG
CSB-MP321620SOG
CSB-EP321620SOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uptake hydrogenase large subunit (hupB), partial CSB-YP321621RKR
CSB-EP321621RKR
CSB-BP321621RKR
CSB-MP321621RKR
CSB-EP321621RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein impA (impA) CSB-YP321622SWX
CSB-EP321622SWX
CSB-BP321622SWX
CSB-MP321622SWX
CSB-EP321622SWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artocarpus integer Agglutinin beta-3 chain CSB-YP321623AOY
CSB-EP321623AOY
CSB-BP321623AOY
CSB-MP321623AOY
CSB-EP321623AOY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage HK022 Transcription termination factor nun (nun) CSB-YP321624ECN
CSB-EP321624ECN
CSB-BP321624ECN
CSB-MP321624ECN
CSB-EP321624ECN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus 60 kDa heat shock protein, mitochondrial (HSPD1), partial CSB-YP321625DXU
CSB-EP321625DXU
CSB-BP321625DXU
CSB-MP321625DXU
CSB-EP321625DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Heat shock 70 kDa protein 2 (UMS2), partial CSB-YP321626UBS
CSB-EP321626UBS
CSB-BP321626UBS
CSB-MP321626UBS
CSB-EP321626UBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein xFG20-1, partial CSB-YP321627XBE
CSB-EP321627XBE
CSB-BP321627XBE
CSB-MP321627XBE
CSB-EP321627XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF44.2 CSB-YP321628XBE
CSB-EP321628XBE
CSB-BP321628XBE
CSB-MP321628XBE
CSB-EP321628XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF52.1 CSB-YP321629XBE
CSB-EP321629XBE
CSB-BP321629XBE
CSB-MP321629XBE
CSB-EP321629XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF66.1, partial CSB-YP321630XBE
CSB-EP321630XBE
CSB-BP321630XBE
CSB-MP321630XBE
CSB-EP321630XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oocyte zinc finger protein XlCOF10 CSB-YP321631XBE
CSB-EP321631XBE
CSB-BP321631XBE
CSB-MP321631XBE
CSB-EP321631XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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