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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Fowlpox virus Protein J1 homolog (FPV133) CSB-YP321405FEY
CSB-EP321405FEY
CSB-BP321405FEY
CSB-MP321405FEY
CSB-EP321405FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar P-ring protein (flgI) CSB-YP321406SXB
CSB-EP321406SXB
CSB-BP321406SXB
CSB-MP321406SXB
CSB-EP321406SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nodulation protein V (nodV), partial CSB-YP321407BVW
CSB-EP321407BVW
CSB-BP321407BVW
CSB-MP321407BVW
CSB-EP321407BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Glutathione S-transferase class-mu 26 kDa isozyme CSB-YP321408SXQ
CSB-EP321408SXQ
CSB-BP321408SXQ
CSB-MP321408SXQ
CSB-EP321408SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelenopsis aperta Omega-agatoxin-Aa2a CSB-YP321409AER
CSB-EP321409AER
CSB-BP321409AER
CSB-MP321409AER
CSB-EP321409AER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Actin-6 (SAC6) CSB-YP321410GGV
CSB-EP321410GGV
CSB-BP321410GGV
CSB-MP321410GGV
CSB-EP321410GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aromatic amino acid transport protein AroP (aroP), partial CSB-YP321411ENV
CSB-EP321411ENV
CSB-BP321411ENV
CSB-MP321411ENV
CSB-EP321411ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP321412ERW1
CSB-EP321412ERW1
CSB-BP321412ERW1
CSB-MP321412ERW1
CSB-EP321412ERW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5 (5), partial CSB-YP321413EDZ
CSB-EP321413EDZ
CSB-BP321413EDZ
CSB-MP321413EDZ
CSB-EP321413EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium sp. Isopenicillin N synthase (pcbC) CSB-YP321414FBW
CSB-EP321414FBW
CSB-BP321414FBW
CSB-MP321414FBW
CSB-EP321414FBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Larix laricina Ribulose bisphosphate carboxylase small chain, chloroplastic (RBCS) CSB-YP321415LCG
CSB-EP321415LCG
CSB-BP321415LCG
CSB-MP321415LCG
CSB-EP321415LCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Chorionic somatomammotropin hormone (CSH) CSB-YP321416SH
CSB-EP321416SH
CSB-BP321416SH
CSB-MP321416SH
CSB-EP321416SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum 15 kDa lipoprotein (tpp15) CSB-YP321417TPR
CSB-EP321417TPR
CSB-BP321417TPR
CSB-MP321417TPR
CSB-EP321417TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus angularis Subtilisin inhibitor 1 CSB-YP321418PGZ
CSB-EP321418PGZ
CSB-BP321418PGZ
CSB-MP321418PGZ
CSB-EP321418PGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-glucosidase A (bglA), partial CSB-YP321419BXZ
CSB-EP321419BXZ
CSB-BP321419BXZ
CSB-MP321419BXZ
CSB-EP321419BXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Threonine synthase (THR4), partial CSB-YP321421SVG
CSB-EP321421SVG
CSB-BP321421SVG
CSB-MP321421SVG
CSB-EP321421SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetabularia cliftonii Ribulose bisphosphate carboxylase small chain 1 (RBCS-1) CSB-YP321422AAW
CSB-EP321422AAW
CSB-BP321422AAW
CSB-MP321422AAW
CSB-EP321422AAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Probable Ni/Fe-hydrogenase B-type cytochrome subunit (hupC), partial CSB-YP321423RLC1
CSB-EP321423RLC1
CSB-BP321423RLC1
CSB-MP321423RLC1
CSB-EP321423RLC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein ERD1 (ERD1), partial CSB-YP321424SVG1
CSB-EP321424SVG1
CSB-BP321424SVG1
CSB-MP321424SVG1
CSB-EP321424SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Anthocyanidin 3-O-glucosyltransferase (BZ1) CSB-YP321425ZAX
CSB-EP321425ZAX
CSB-BP321425ZAX
CSB-MP321425ZAX
CSB-EP321425ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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