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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Sporulation-specific wall maturation protein (SPS100) CSB-YP321067SVG
CSB-EP321067SVG
CSB-BP321067SVG
CSB-MP321067SVG
CSB-EP321067SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP321068IFH
CSB-EP321068IFH
CSB-BP321068IFH
CSB-MP321068IFH
CSB-EP321068IFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP321069IFC
CSB-EP321069IFC
CSB-BP321069IFC
CSB-MP321069IFC
CSB-EP321069IFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem I reaction center subunit VIII (psaI), partial CSB-YP321070HWQ
CSB-EP321070HWQ
CSB-BP321070HWQ
CSB-MP321070HWQ
CSB-EP321070HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP321071IMF
CSB-EP321071IMF
CSB-BP321071IMF
CSB-MP321071IMF
CSB-EP321071IMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP321072IFU
CSB-EP321072IFU
CSB-BP321072IFU
CSB-MP321072IFU
CSB-EP321072IFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP321073IGX
CSB-EP321073IGX
CSB-BP321073IGX
CSB-MP321073IGX
CSB-EP321073IGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP321074IIA
CSB-EP321074IIA
CSB-BP321074IIA
CSB-MP321074IIA
CSB-EP321074IIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glutamine--tRNA ligase (GLN4), partial CSB-YP321075SVG
CSB-EP321075SVG
CSB-BP321075SVG
CSB-MP321075SVG
CSB-EP321075SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem I reaction center subunit III, chloroplastic (PSAF) CSB-YP321076HWQ
CSB-EP321076HWQ
CSB-BP321076HWQ
CSB-MP321076HWQ
CSB-EP321076HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Envelope glycoprotein B (gB), partial CSB-YP321077HWX
CSB-EP321077HWX
CSB-BP321077HWX
CSB-MP321077HWX
CSB-EP321077HWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Tryptophan synthase (trp-3), partial CSB-YP321078NHA
CSB-EP321078NHA
CSB-BP321078NHA
CSB-MP321078NHA
CSB-EP321078NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable fructose-bisphosphate aldolase (fbaA) CSB-YP321079BRJ
CSB-EP321079BRJ
CSB-BP321079BRJ
CSB-MP321079BRJ
CSB-EP321079BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida L-methionine gamma-lyase (mdeA) CSB-YP321081FFZ
CSB-EP321081FFZ
CSB-BP321081FFZ
CSB-MP321081FFZ
CSB-EP321081FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase large chain (carB) CSB-YP321082MSL
CSB-EP321082MSL
CSB-BP321082MSL
CSB-MP321082MSL
CSB-EP321082MSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA polymerase III polC-type (polC), partial CSB-YP321083BRJ
CSB-EP321083BRJ
CSB-BP321083BRJ
CSB-MP321083BRJ
CSB-EP321083BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Digestive cysteine proteinase 1 (LCP1) CSB-YP321084HWO
CSB-EP321084HWO
CSB-BP321084HWO
CSB-MP321084HWO
CSB-EP321084HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echinus esculentus Histone H2B.1, sperm CSB-YP321085EAG
CSB-EP321085EAG
CSB-BP321085EAG
CSB-MP321085EAG
CSB-EP321085EAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Envelope glycoprotein G (gG), partial CSB-YP321087HJX1
CSB-EP321087HJX1
CSB-BP321087HJX1
CSB-MP321087HJX1
CSB-EP321087HJX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 14.6 kDa protein in mobD-ri intergenic region (y05O) CSB-YP321088EDZ
CSB-EP321088EDZ
CSB-BP321088EDZ
CSB-MP321088EDZ
CSB-EP321088EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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