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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Guillardia theta 30S ribosomal protein S7, chloroplastic (rps7) CSB-YP321693GHG
CSB-EP321693GHG
CSB-BP321693GHG
CSB-MP321693GHG
CSB-EP321693GHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Rev (rev) CSB-YP321694SHD
CSB-EP321694SHD
CSB-BP321694SHD
CSB-MP321694SHD
CSB-EP321694SHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Tat (tat) CSB-YP321695SHD
CSB-EP321695SHD
CSB-BP321695SHD
CSB-MP321695SHD
CSB-EP321695SHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipase CSB-YP321696RDA
CSB-BP321696RDA
CSB-MP321696RDA
CSB-EP321696RDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Maltogenic alpha-amylase (amyM), partial CSB-YP321697GFM
CSB-EP321697GFM
CSB-BP321697GFM
CSB-MP321697GFM
CSB-EP321697GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef) CSB-YP321698HLD
CSB-EP321698HLD
CSB-BP321698HLD
CSB-MP321698HLD
CSB-EP321698HLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP321699HLE
CSB-EP321699HLE
CSB-BP321699HLE
CSB-MP321699HLE
CSB-EP321699HLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine immunodeficiency virus Envelope glycoprotein (env), partial CSB-YP321700BKB
CSB-EP321700BKB
CSB-BP321700BKB
CSB-MP321700BKB
CSB-EP321700BKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Maltose/maltodextrin import ATP-binding protein MalK (malK) CSB-YP321701SXB
CSB-EP321701SXB
CSB-BP321701SXB
CSB-MP321701SXB
CSB-EP321701SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Nitrous-oxide reductase (nosZ), partial CSB-YP321702FGJ
CSB-EP321702FGJ
CSB-BP321702FGJ
CSB-MP321702FGJ
CSB-EP321702FGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsule biosynthesis protein CapA (capA), partial CSB-YP321703BQE1
CSB-EP321703BQE1
CSB-BP321703BQE1
CSB-MP321703BQE1
CSB-EP321703BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Oxygen-evolving enhancer protein 3 (PSBQ) CSB-YP321704EWE
CSB-EP321704EWE
CSB-BP321704EWE
CSB-MP321704EWE
CSB-EP321704EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Heterogeneous nuclear ribonucleoprotein C (hnrnpc) CSB-YP321705XBE
CSB-EP321705XBE
CSB-BP321705XBE
CSB-MP321705XBE
CSB-EP321705XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrozoarces americanus Ice-structuring protein lambda OP-5 CSB-YP321706MAQ
CSB-EP321706MAQ
CSB-BP321706MAQ
CSB-MP321706MAQ
CSB-EP321706MAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Major yolk protein, partial CSB-YP321707FOT
CSB-EP321707FOT
CSB-BP321707FOT
CSB-MP321707FOT
CSB-EP321707FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Treponemal membrane protein B (tmpB) CSB-YP321708TPR
CSB-EP321708TPR
CSB-BP321708TPR
CSB-MP321708TPR
CSB-EP321708TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P1 Protein kilA (kilA) CSB-YP321709EDD
CSB-EP321709EDD
CSB-BP321709EDD
CSB-MP321709EDD
CSB-EP321709EDD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridine phosphorylase (udp) CSB-YP321710LMW
CSB-EP321710LMW
CSB-BP321710LMW
CSB-MP321710LMW
CSB-EP321710LMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molineria latifolia Curculin-1 (CUR09) CSB-YP321711CUT
CSB-EP321711CUT
CSB-BP321711CUT
CSB-MP321711CUT
CSB-EP321711CUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana sylvestris 31 kDa ribonucleoprotein, chloroplastic CSB-YP321712NHD
CSB-EP321712NHD
CSB-BP321712NHD
CSB-MP321712NHD
CSB-EP321712NHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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