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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Protein kinase DC2 (Pka-C3), partial CSB-YP321489DLU
CSB-EP321489DLU
CSB-BP321489DLU
CSB-MP321489DLU
CSB-EP321489DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conjugation stage-specific protein (CNJC) CSB-YP321490TLU
CSB-EP321490TLU
CSB-BP321490TLU
CSB-MP321490TLU
CSB-EP321490TLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS600 uncharacterized 11 kDa protein CSB-YP321491SGH
CSB-EP321491SGH
CSB-BP321491SGH
CSB-MP321491SGH
CSB-EP321491SGH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant M protein, serotype 49 (emm49) CSB-YP321492SMX
CSB-EP321492SMX
CSB-BP321492SMX
CSB-MP321492SMX
CSB-EP321492SMX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ferredoxin-2, chloroplastic (FD2) CSB-YP321493DOA
CSB-EP321493DOA
CSB-BP321493DOA
CSB-MP321493DOA
CSB-EP321493DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP321494IGR
CSB-EP321494IGR
CSB-BP321494IGR
CSB-MP321494IGR
CSB-EP321494IGR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP321495IDQ
CSB-EP321495IDQ
CSB-BP321495IDQ
CSB-MP321495IDQ
CSB-EP321495IDQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial CSB-YP321496IKJ
CSB-EP321496IKJ
CSB-BP321496IKJ
CSB-MP321496IKJ
CSB-EP321496IKJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 12 (ZNF12) CSB-YP321497HU
CSB-EP321497HU
CSB-BP321497HU
CSB-MP321497HU
CSB-EP321497HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP321498IEY
CSB-EP321498IEY
CSB-BP321498IEY
CSB-MP321498IEY
CSB-EP321498IEY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant G surface protein, allelic form 168 (168G), partial CSB-YP321499ERV
CSB-EP321499ERV
CSB-BP321499ERV
CSB-MP321499ERV
CSB-EP321499ERV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L8-A (RPL8A) CSB-YP321500SVG
CSB-EP321500SVG
CSB-BP321500SVG
CSB-MP321500SVG
CSB-EP321500SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Urease subunit gamma (ureA) CSB-YP321501EYZ
CSB-EP321501EYZ
CSB-BP321501EYZ
CSB-MP321501EYZ
CSB-EP321501EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype A2 subtype adw2 Protein X (X) CSB-YP321502HDO
CSB-EP321502HDO
CSB-BP321502HDO
CSB-MP321502HDO
CSB-EP321502HDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Arabinose 5-phosphate isomerase GutQ (gutQ) CSB-YP321503ENV
CSB-EP321503ENV
CSB-BP321503ENV
CSB-MP321503ENV
CSB-EP321503ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein J1S CSB-YP321504HWV
CSB-EP321504HWV
CSB-BP321504HWV
CSB-MP321504HWV
CSB-EP321504HWV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein Z (psbZ), partial CSB-YP321505DZX1
CSB-EP321505DZX1
CSB-BP321505DZX1
CSB-MP321505DZX1
CSB-EP321505DZX1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase alpha chain (trpA) CSB-YP321506LMH
CSB-EP321506LMH
CSB-BP321506LMH
CSB-MP321506LMH
CSB-EP321506LMH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Early glycoprotein GP48 (UL4) CSB-YP321507HWX
CSB-EP321507HWX
CSB-BP321507HWX
CSB-MP321507HWX
CSB-EP321507HWX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 1-alpha (tuf) CSB-YP321508TDE
CSB-EP321508TDE
CSB-BP321508TDE
CSB-MP321508TDE
CSB-EP321508TDE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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