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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter aerogenes Para-aminobenzoate synthase component 1 (pabB) CSB-YP321005EKN
CSB-EP321005EKN
CSB-BP321005EKN
CSB-MP321005EKN
CSB-EP321005EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Phosphoglycerate transporter protein (pgtP), partial CSB-YP321006SXB
CSB-EP321006SXB
CSB-BP321006SXB
CSB-MP321006SXB
CSB-EP321006SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase YPK1 (YPK1), partial CSB-YP321007SVG
CSB-EP321007SVG
CSB-BP321007SVG
CSB-MP321007SVG
CSB-EP321007SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium ATP synthase epsilon chain (atpC) CSB-YP321008BQW
CSB-EP321008BQW
CSB-BP321008BQW
CSB-MP321008BQW
CSB-EP321008BQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisaster ochraceus Actin, cytoplasmic CSB-YP321009PKW
CSB-EP321009PKW
CSB-BP321009PKW
CSB-MP321009PKW
CSB-EP321009PKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Alpha-1-antiproteinase CSB-YP321010SH
CSB-EP321010SH
CSB-BP321010SH
CSB-MP321010SH
CSB-EP321010SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S19 (rpsS) CSB-YP321011GFM
CSB-EP321011GFM
CSB-BP321011GFM
CSB-MP321011GFM
CSB-EP321011GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 50S ribosomal protein L4P (rpl4p) CSB-YP321012HTJ
CSB-EP321012HTJ
CSB-BP321012HTJ
CSB-MP321012HTJ
CSB-EP321012HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S8P (rps8p) CSB-YP321013HTJ
CSB-EP321013HTJ
CSB-BP321013HTJ
CSB-MP321013HTJ
CSB-EP321013HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio fischeri Transcriptional activator protein luxR (luxR) CSB-YP321014VDZ
CSB-EP321014VDZ
CSB-BP321014VDZ
CSB-MP321014VDZ
CSB-EP321014VDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uridine phosphorylase (udp) CSB-YP321016ENV
CSB-EP321016ENV
CSB-BP321016ENV
CSB-MP321016ENV
CSB-EP321016ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Deoxyribodipyrimidine photo-lyase (phr) CSB-YP321017MSR
CSB-EP321017MSR
CSB-BP321017MSR
CSB-MP321017MSR
CSB-EP321017MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Phosphoglycerate kinase, chloroplastic, partial CSB-YP321018TQN
CSB-EP321018TQN
CSB-BP321018TQN
CSB-MP321018TQN
CSB-EP321018TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sandfly fever sicilian virus Nucleoprotein (N) CSB-YP321019SCE
CSB-BP321019SCE
CSB-MP321019SCE
CSB-EP321019SCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum 16.9 kDa class I heat shock protein 1 (hsp16.9A) CSB-YP321020TQN
CSB-EP321020TQN
CSB-BP321020TQN
CSB-MP321020TQN
CSB-EP321020TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Protamine-2A CSB-YP321021OEI
CSB-EP321021OEI
CSB-BP321021OEI
CSB-MP321021OEI
CSB-EP321021OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Early 18.5 kDa protein (DA18) CSB-YP321022ARA
CSB-EP321022ARA
CSB-BP321022ARA
CSB-MP321022ARA
CSB-EP321022ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 60 kDa chaperonin 1 (groL1), partial CSB-YP321023FPZ
CSB-EP321023FPZ
CSB-BP321023FPZ
CSB-MP321023FPZ
CSB-EP321023FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Muraenesox cinereus Gonadotropin subunit beta (cgb) CSB-YP321024MJQ
CSB-EP321024MJQ
CSB-BP321024MJQ
CSB-MP321024MJQ
CSB-EP321024MJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose isomerase (xylA) CSB-YP321025ACX
CSB-EP321025ACX
CSB-BP321025ACX
CSB-MP321025ACX
CSB-EP321025ACX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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