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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhizobium radiobacter Isopentenyl transferase (ipt) CSB-YP321385RKV
CSB-EP321385RKV
CSB-BP321385RKV
CSB-MP321385RKV
CSB-EP321385RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP321386ILL
CSB-EP321386ILL
CSB-BP321386ILL
CSB-MP321386ILL
CSB-EP321386ILL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP321387IEI
CSB-EP321387IEI
CSB-BP321387IEI
CSB-MP321387IEI
CSB-EP321387IEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP321388IIK
CSB-EP321388IIK
CSB-BP321388IIK
CSB-MP321388IIK
CSB-EP321388IIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-keto-3-deoxygluconate permease (kdgT), partial CSB-YP321389EGI
CSB-EP321389EGI
CSB-BP321389EGI
CSB-MP321389EGI
CSB-EP321389EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Phosphate-repressible phosphate permease (pho-4), partial CSB-YP321390NHA
CSB-EP321390NHA
CSB-BP321390NHA
CSB-MP321390NHA
CSB-EP321390NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Malate dehydrogenase [NADP], chloroplastic CSB-YP321391ZAX
CSB-EP321391ZAX
CSB-BP321391ZAX
CSB-MP321391ZAX
CSB-EP321391ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein O (nodO) CSB-YP321392RKR
CSB-EP321392RKR
CSB-BP321392RKR
CSB-MP321392RKR
CSB-EP321392RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type II secretion system protein F (pulF), partial CSB-YP321393KBG1
CSB-EP321393KBG1
CSB-BP321393KBG1
CSB-MP321393KBG1
CSB-EP321393KBG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type II secretion system protein N (pulN), partial CSB-YP321394KBG
CSB-EP321394KBG
CSB-BP321394KBG
CSB-MP321394KBG
CSB-EP321394KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L3, cyanelle (rpl3) CSB-YP321395DZX
CSB-EP321395DZX
CSB-BP321395DZX
CSB-MP321395DZX
CSB-EP321395DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Shikimate dehydrogenase (aroE) CSB-YP321396ENV
CSB-EP321396ENV
CSB-BP321396ENV
CSB-MP321396ENV
CSB-EP321396ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella choleraesuis 27.5 kDa virulence protein (spvC) CSB-YP321397SBF
CSB-EP321397SBF
CSB-BP321397SBF
CSB-MP321397SBF
CSB-EP321397SBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vicia faba Protein ycf2 (ycf2) CSB-YP321398VFJ
CSB-EP321398VFJ
CSB-BP321398VFJ
CSB-MP321398VFJ
CSB-EP321398VFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Acidic ribosomal protein P0 homolog (rplP0) CSB-YP321399MNS
CSB-EP321399MNS
CSB-BP321399MNS
CSB-MP321399MNS
CSB-EP321399MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Virion infectivity factor (vif) CSB-YP321400HMD
CSB-EP321400HMD
CSB-BP321400HMD
CSB-MP321400HMD
CSB-EP321400HMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Calcium-binding protein SPEC 2D (SPEC2D) CSB-YP321401FOT
CSB-EP321401FOT
CSB-BP321401FOT
CSB-MP321401FOT
CSB-EP321401FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Homeobox protein H55 CSB-YP321402DNK
CSB-EP321402DNK
CSB-BP321402DNK
CSB-MP321402DNK
CSB-EP321402DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone H1C CSB-YP321403XBE
CSB-EP321403XBE
CSB-BP321403XBE
CSB-MP321403XBE
CSB-EP321403XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-R8A2 B Uncharacterized protein ORF6 (ORF6), partial CSB-YP321404SJZ1
CSB-EP321404SJZ1
CSB-BP321404SJZ1
CSB-MP321404SJZ1
CSB-EP321404SJZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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