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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP321713YAK
CSB-EP321713YAK
CSB-BP321713YAK
CSB-MP321713YAK
CSB-EP321713YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP321714IEI
CSB-EP321714IEI
CSB-BP321714IEI
CSB-MP321714IEI
CSB-EP321714IEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP321715IKH
CSB-EP321715IKH
CSB-BP321715IKH
CSB-MP321715IKH
CSB-EP321715IKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E6 (E6) CSB-YP321716HNP
CSB-EP321716HNP
CSB-BP321716HNP
CSB-MP321716HNP
CSB-EP321716HNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Phosphoprotein (P/V) CSB-YP321717MJM
CSB-EP321717MJM
CSB-BP321717MJM
CSB-MP321717MJM
CSB-EP321717MJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine adenovirus A serotype 1 Early E3 17.7 kDa glycoprotein, partial CSB-YP321718MUS1
CSB-EP321718MUS1
CSB-BP321718MUS1
CSB-MP321718MUS1
CSB-EP321718MUS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T7 Minor capsid protein 10B (10) CSB-YP321719EEB
CSB-EP321719EEB
CSB-BP321719EEB
CSB-MP321719EEB
CSB-EP321719EEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Phenol hydroxylase P2 protein (dmpM) CSB-YP321720PVK
CSB-EP321720PVK
CSB-BP321720PVK
CSB-MP321720PVK
CSB-EP321720PVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor PRP9 (PRP9), partial CSB-YP321721SVG
CSB-EP321721SVG
CSB-BP321721SVG
CSB-MP321721SVG
CSB-EP321721SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline coronavirus Non-structural protein 7a (7a), partial CSB-YP321722FEJ
CSB-BP321722FEJ
CSB-MP321722FEJ
CSB-EP321722FEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheeppox virus Probable host range protein 2 (CF8A) CSB-YP321723SFW
CSB-EP321723SFW
CSB-BP321723SFW
CSB-MP321723SFW
CSB-EP321723SFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Hemagglutinin-neuraminidase (HN), partial CSB-YP321724SEX1
CSB-EP321724SEX1
CSB-BP321724SEX1
CSB-MP321724SEX1
CSB-EP321724SEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS1 protein insA (insA) CSB-YP321725SZB
CSB-EP321725SZB
CSB-BP321725SZB
CSB-MP321725SZB
CSB-EP321725SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS986 uncharacterized 8.2 kDa protein CSB-YP321726MVZ
CSB-EP321726MVZ
CSB-BP321726MVZ
CSB-MP321726MVZ
CSB-EP321726MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS110 uncharacterized 11.7 kDa protein CSB-YP321727SNZ
CSB-EP321727SNZ
CSB-BP321727SNZ
CSB-MP321727SNZ
CSB-EP321727SNZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum N- (5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase, partial CSB-YP321728ETG
CSB-EP321728ETG
CSB-BP321728ETG
CSB-MP321728ETG
CSB-EP321728ETG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis FMRFamide neuropeptides CSB-YP321729LRQ
CSB-EP321729LRQ
CSB-BP321729LRQ
CSB-MP321729LRQ
CSB-EP321729LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Crustacean hyperglycemic hormones isoform A CSB-YP321730HWO
CSB-EP321730HWO
CSB-BP321730HWO
CSB-MP321730HWO
CSB-EP321730HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Trans-Golgi network integral membrane protein TGN38 (Ttgn1), partial CSB-YP321731RA
CSB-EP321731RA
CSB-BP321731RA
CSB-MP321731RA
CSB-EP321731RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera argillicola Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP321732ODY1
CSB-EP321732ODY1
CSB-BP321732ODY1
CSB-MP321732ODY1
CSB-EP321732ODY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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