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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii 10 kDa chaperonin (groS) CSB-YP322529DXP
CSB-EP322529DXP
CSB-BP322529DXP
CSB-MP322529DXP
CSB-EP322529DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nodulation protein S (nodS) CSB-YP322530RKX
CSB-EP322530RKX
CSB-BP322530RKX
CSB-MP322530RKX
CSB-EP322530RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter xylinus Cellulose synthase catalytic subunit [UDP-forming] (bcsA), partial CSB-YP322531GGT
CSB-EP322531GGT
CSB-BP322531GGT
CSB-MP322531GGT
CSB-EP322531GGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 25 kDa ookinete surface antigen CSB-YP322532PMP
CSB-EP322532PMP
CSB-BP322532PMP
CSB-MP322532PMP
CSB-EP322532PMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guillardia theta 30S ribosomal protein S10, chloroplastic (rps10) CSB-YP322533GHG
CSB-EP322533GHG
CSB-BP322533GHG
CSB-MP322533GHG
CSB-EP322533GHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Persea americana 1-aminocyclopropane-1-carboxylate oxidase (ACO) CSB-YP322534PGI
CSB-EP322534PGI
CSB-BP322534PGI
CSB-MP322534PGI
CSB-EP322534PGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum Eggshell protein 1 (ESG-1) CSB-YP322535SXP
CSB-EP322535SXP
CSB-BP322535SXP
CSB-MP322535SXP
CSB-EP322535SXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum 34 kDa membrane antigen (tpd) CSB-YP322536TPR
CSB-BP322536TPR
CSB-MP322536TPR
CSB-EP322536TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Major extracellular endoglucanase (engXCA) CSB-YP322537XAY
CSB-EP322537XAY
CSB-BP322537XAY
CSB-MP322537XAY
CSB-EP322537XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Coenzyme F420 hydrogenase subunit alpha (frhA) CSB-YP322538MSR
CSB-EP322538MSR
CSB-BP322538MSR
CSB-MP322538MSR
CSB-EP322538MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Nef (nef) CSB-YP322539SHD
CSB-EP322539SHD
CSB-BP322539SHD
CSB-MP322539SHD
CSB-EP322539SHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovine maedi visna related virus Putative uncharacterized protein W CSB-YP322540OCW
CSB-EP322540OCW
CSB-BP322540OCW
CSB-MP322540OCW
CSB-EP322540OCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane porin, serovar L1 (ompA) CSB-YP322541DSA
CSB-EP322541DSA
CSB-BP322541DSA
CSB-MP322541DSA
CSB-EP322541DSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP322542HLD
CSB-EP322542HLD
CSB-BP322542HLD
CSB-MP322542HLD
CSB-EP322542HLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP322543HLD
CSB-EP322543HLD
CSB-BP322543HLD
CSB-MP322543HLD
CSB-EP322543HLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudoazurin (bcp) CSB-YP322544ABL
CSB-EP322544ABL
CSB-BP322544ABL
CSB-MP322544ABL
CSB-EP322544ABL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Diaminopimelate decarboxylase (lysA) CSB-YP322545EZX
CSB-EP322545EZX
CSB-BP322545EZX
CSB-MP322545EZX
CSB-EP322545EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsule biosynthesis protein CapB (capB), partial CSB-YP322546BQE1
CSB-EP322546BQE1
CSB-BP322546BQE1
CSB-MP322546BQE1
CSB-EP322546BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP322547SDI1
CSB-EP322547SDI1
CSB-BP322547SDI1
CSB-MP322547SDI1
CSB-EP322547SDI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrozoarces americanus Ice-structuring protein SP1 (HPLC 4) CSB-YP322548MAQ
CSB-EP322548MAQ
CSB-BP322548MAQ
CSB-MP322548MAQ
CSB-EP322548MAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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