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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila melanogaster Tyramine/octopamine receptor (Oct-TyrR), partial CSB-YP322124DLU1
CSB-EP322124DLU1
CSB-BP322124DLU1
CSB-MP322124DLU1
CSB-EP322124DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physarum polycephalum Profilin-A (PROA) CSB-YP322125EVC
CSB-EP322125EVC
CSB-BP322125EVC
CSB-MP322125EVC
CSB-EP322125EVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor circinelloides f. lusitanicus Ras-like protein 3 (RAS3) CSB-YP322126MIW
CSB-EP322126MIW
CSB-BP322126MIW
CSB-MP322126MIW
CSB-EP322126MIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Poa pratensis Pollen allergen KBG 31 CSB-YP322127PNP
CSB-EP322127PNP
CSB-BP322127PNP
CSB-MP322127PNP
CSB-EP322127PNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome b-c1 complex subunit 9 (QCR9), partial CSB-YP322128SVG1
CSB-EP322128SVG1
CSB-BP322128SVG1
CSB-MP322128SVG1
CSB-EP322128SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax Duffy receptor (PVDR), partial CSB-YP322129EWW
CSB-EP322129EWW
CSB-BP322129EWW
CSB-MP322129EWW
CSB-EP322129EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sciara coprophila Puff II/9-1 protein (II/9-1) CSB-YP322131SDT
CSB-EP322131SDT
CSB-BP322131SDT
CSB-MP322131SDT
CSB-EP322131SDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sciara coprophila Puff II/9-2 protein (II/9-2) CSB-YP322132SDT
CSB-EP322132SDT
CSB-BP322132SDT
CSB-MP322132SDT
CSB-EP322132SDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator NAD-reducing hydrogenase hoxS subunit delta (hoxY) CSB-YP322133DZQ
CSB-EP322133DZQ
CSB-BP322133DZQ
CSB-MP322133DZQ
CSB-EP322133DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium Germination protease (gpr) CSB-YP322134BQW
CSB-EP322134BQW
CSB-BP322134BQW
CSB-MP322134BQW
CSB-EP322134BQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Alpha-1-antiproteinase F CSB-YP322135GU
CSB-EP322135GU
CSB-BP322135GU
CSB-MP322135GU
CSB-EP322135GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplusia ni Acidic juvenile hormone-suppressible protein 1 (AJSP-1), partial CSB-YP322136TQE
CSB-EP322136TQE
CSB-BP322136TQE
CSB-MP322136TQE
CSB-EP322136TQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astyanax fasciatus Green-sensitive opsin-1 (G103), partial CSB-YP322137DPH1
CSB-EP322137DPH1
CSB-BP322137DPH1
CSB-MP322137DPH1
CSB-EP322137DPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Replication factor A protein 1 (RFA1), partial CSB-YP322138SVG
CSB-EP322138SVG
CSB-BP322138SVG
CSB-MP322138SVG
CSB-EP322138SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Putative glutamine amidotransferase-like protein yvdE homolog CSB-YP322139LNF
CSB-EP322139LNF
CSB-BP322139LNF
CSB-MP322139LNF
CSB-EP322139LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bursal disease virus Structural polyprotein, partial CSB-YP322141ASK
CSB-EP322141ASK
CSB-BP322141ASK
CSB-MP322141ASK
CSB-EP322141ASK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 54S ribosomal protein L20, mitochondrial (MRPL20) CSB-YP322142SVG
CSB-EP322142SVG
CSB-BP322142SVG
CSB-MP322142SVG
CSB-EP322142SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Chaperone protein dnaK2 (dnaK2), partial CSB-YP322143SSQ
CSB-EP322143SSQ
CSB-BP322143SSQ
CSB-MP322143SSQ
CSB-EP322143SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amicyanin (mauC) CSB-YP322144PBZ
CSB-EP322144PBZ
CSB-BP322144PBZ
CSB-MP322144PBZ
CSB-EP322144PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium patulum 6-methylsalicylic acid synthase, partial CSB-YP322145PFB
CSB-EP322145PFB
CSB-BP322145PFB
CSB-MP322145PFB
CSB-EP322145PFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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