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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacteria phage T3 Bacterial RNA polymerase inhibitor (2) CSB-YP321849EDY
CSB-EP321849EDY
CSB-BP321849EDY
CSB-MP321849EDY
CSB-EP321849EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Putative serine proteinase inhibitor 2 homolog second part (B14R) CSB-YP321850VAA
CSB-EP321850VAA
CSB-BP321850VAA
CSB-MP321850VAA
CSB-EP321850VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fanG (fanG) CSB-YP321851ENL
CSB-EP321851ENL
CSB-BP321851ENL
CSB-MP321851ENL
CSB-EP321851ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fanH (fanH) CSB-YP321852ENL
CSB-EP321852ENL
CSB-BP321852ENL
CSB-MP321852ENL
CSB-EP321852ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Nef (nef) CSB-YP321853HMB
CSB-EP321853HMB
CSB-BP321853HMB
CSB-MP321853HMB
CSB-EP321853HMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag-Pol polyprotein (gag-pol), partial CSB-YP321854HKS
CSB-EP321854HKS
CSB-BP321854HKS
CSB-MP321854HKS
CSB-EP321854HKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpu (vpu), partial CSB-YP321855HKS
CSB-EP321855HKS
CSB-BP321855HKS
CSB-MP321855HKS
CSB-EP321855HKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP321856HKZ
CSB-EP321856HKZ
CSB-BP321856HKZ
CSB-MP321856HKZ
CSB-EP321856HKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP321857HKZ
CSB-EP321857HKZ
CSB-BP321857HKZ
CSB-MP321857HKZ
CSB-EP321857HKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus ruber ruber Zinc metalloproteinase HT-2 CSB-YP321858CTD
CSB-EP321858CTD
CSB-BP321858CTD
CSB-MP321858CTD
CSB-EP321858CTD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cysteine proteinase inhibitor 2 (Os05g0494200, LOC_Os05g41460) CSB-YP321859OFG
CSB-EP321859OFG
CSB-BP321859OFG
CSB-MP321859OFG
CSB-EP321859OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine infectious anemia virus Protein Tat (tat) CSB-YP321860EFZ
CSB-EP321860EFZ
CSB-BP321860EFZ
CSB-MP321860EFZ
CSB-EP321860EFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable hydrogenase nickel incorporation protein HybF (hybF) CSB-YP321861EZA
CSB-EP321861EZA
CSB-BP321861EZA
CSB-MP321861EZA
CSB-EP321861EZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Regulatory protein abaA (abaA), partial CSB-YP321862EKF
CSB-EP321862EKF
CSB-BP321862EKF
CSB-MP321862EKF
CSB-EP321862EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaya mosaic potexvirus RNA replication protein, partial CSB-YP321863PBN
CSB-EP321863PBN
CSB-BP321863PBN
CSB-MP321863PBN
CSB-EP321863PBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaya mosaic potexvirus Movement protein TGBp3 (ORF4), partial CSB-YP321864PBN
CSB-EP321864PBN
CSB-BP321864PBN
CSB-MP321864PBN
CSB-EP321864PBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrilase, arylacetone-specific CSB-YP321865AGN
CSB-EP321865AGN
CSB-BP321865AGN
CSB-MP321865AGN
CSB-EP321865AGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 2-oxoglutarate dehydrogenase, mitochondrial (KGD1), partial CSB-YP321866SVG
CSB-EP321866SVG
CSB-BP321866SVG
CSB-MP321866SVG
CSB-EP321866SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerate kinase (pgk) CSB-YP321867MRN
CSB-EP321867MRN
CSB-BP321867MRN
CSB-MP321867MRN
CSB-EP321867MRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A56 (HA), partial CSB-YP321868VAA1
CSB-BP321868VAA1
CSB-MP321868VAA1
CSB-EP321868VAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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