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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Magnesium-chelatase subunit chlI, chloroplastic (CHLI) CSB-YP322829DOA
CSB-EP322829DOA
CSB-BP322829DOA
CSB-MP322829DOA
CSB-EP322829DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 41 Minor capsid protein 6 (PVI) CSB-YP322830HIS
CSB-EP322830HIS
CSB-BP322830HIS
CSB-MP322830HIS
CSB-EP322830HIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis POU domain, class 2, transcription factor 1 (pou2f1), partial CSB-YP322831XBE
CSB-EP322831XBE
CSB-BP322831XBE
CSB-MP322831XBE
CSB-EP322831XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Anthocyanidin 3-O-glucosyltransferase (BZ1) CSB-YP322832ZAX
CSB-EP322832ZAX
CSB-BP322832ZAX
CSB-MP322832ZAX
CSB-EP322832ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Glycoprotein NB (NB), partial CSB-YP322833IJM1
CSB-EP322833IJM1
CSB-BP322833IJM1
CSB-MP322833IJM1
CSB-EP322833IJM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Neuraminidase (NA), partial CSB-YP322834IJV
CSB-EP322834IJV
CSB-BP322834IJV
CSB-MP322834IJV
CSB-EP322834IJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Locustatachykinin-2 CSB-YP322835LHY
CSB-EP322835LHY
CSB-BP322835LHY
CSB-MP322835LHY
CSB-EP322835LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum peruvianum Wound-induced proteinase inhibitor 1 CSB-YP322836FIF
CSB-EP322836FIF
CSB-BP322836FIF
CSB-MP322836FIF
CSB-EP322836FIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Protein RecA (recA) CSB-YP322837TOA
CSB-EP322837TOA
CSB-BP322837TOA
CSB-MP322837TOA
CSB-EP322837TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Histidinol-phosphate aminotransferase (hisC) CSB-YP322838FOB
CSB-EP322838FOB
CSB-BP322838FOB
CSB-MP322838FOB
CSB-EP322838FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron-iron protein beta chain (anfK) CSB-YP322839DPX
CSB-EP322839DPX
CSB-BP322839DPX
CSB-MP322839DPX
CSB-EP322839DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA ligase (LIG), partial CSB-YP322840VAI
CSB-EP322840VAI
CSB-BP322840VAI
CSB-MP322840VAI
CSB-EP322840VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin alpha-B chain (apcD) CSB-YP322841DZX
CSB-EP322841DZX
CSB-BP322841DZX
CSB-MP322841DZX
CSB-EP322841DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glutenin, low molecular weight subunit PTDUCD1 CSB-YP322842TQN
CSB-EP322842TQN
CSB-BP322842TQN
CSB-MP322842TQN
CSB-EP322842TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar hook-associated protein 3 (flgL) CSB-YP322843SXB
CSB-EP322843SXB
CSB-BP322843SXB
CSB-MP322843SXB
CSB-EP322843SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agkistrodon piscivorus piscivorus Disintegrin applagin CSB-YP322844AYP
CSB-EP322844AYP
CSB-BP322844AYP
CSB-MP322844AYP
CSB-EP322844AYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Endogenous alpha-amylase/subtilisin inhibitor CSB-YP322845TQN
CSB-EP322845TQN
CSB-BP322845TQN
CSB-MP322845TQN
CSB-EP322845TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heloderma suspectum Phospholipase A2 isozymes PA3A/PA3B/PA5 CSB-YP322846HVJ
CSB-EP322846HVJ
CSB-BP322846HVJ
CSB-MP322846HVJ
CSB-EP322846HVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Steroid receptor seven-up, isoforms B/C (svp), partial CSB-YP322847DLU
CSB-EP322847DLU
CSB-BP322847DLU
CSB-MP322847DLU
CSB-EP322847DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat RT1 class I histocompatibility antigen, AA alpha chain, partial CSB-YP322848RA
CSB-EP322848RA
CSB-BP322848RA
CSB-MP322848RA
CSB-EP322848RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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