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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Sporulation protein ydcC (ydcC), partial CSB-YP311398BRJ
CSB-EP311398BRJ
CSB-BP311398BRJ
CSB-MP311398BRJ
CSB-EP311398BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein sprT-like (ydcK) CSB-YP311399BRJ
CSB-EP311399BRJ
CSB-BP311399BRJ
CSB-MP311399BRJ
CSB-EP311399BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddA (yddA) CSB-YP311400BRJ
CSB-EP311400BRJ
CSB-BP311400BRJ
CSB-MP311400BRJ
CSB-EP311400BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddF (yddF) CSB-YP311401BRJ
CSB-EP311401BRJ
CSB-BP311401BRJ
CSB-MP311401BRJ
CSB-EP311401BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddK (yddK) CSB-YP311402BRJ
CSB-EP311402BRJ
CSB-BP311402BRJ
CSB-MP311402BRJ
CSB-EP311402BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydeC (ydeC) CSB-YP311403BRJ
CSB-EP311403BRJ
CSB-BP311403BRJ
CSB-MP311403BRJ
CSB-EP311403BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized NAD (P)H oxidoreductase ydeQ CSB-YP311404BRJ
CSB-EP311404BRJ
CSB-BP311404BRJ
CSB-MP311404BRJ
CSB-EP311404BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydfF (ydfF) CSB-YP311405BRJ
CSB-EP311405BRJ
CSB-BP311405BRJ
CSB-MP311405BRJ
CSB-EP311405BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized carboxylesterase nap (nap) CSB-YP311406BRJ
CSB-EP311406BRJ
CSB-BP311406BRJ
CSB-MP311406BRJ
CSB-EP311406BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thioredoxin-like protein ydfQ (ydfQ) CSB-YP311407BRJ
CSB-EP311407BRJ
CSB-BP311407BRJ
CSB-MP311407BRJ
CSB-EP311407BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YdgE (ydgE) CSB-YP311408BRJ
CSB-EP311408BRJ
CSB-BP311408BRJ
CSB-MP311408BRJ
CSB-EP311408BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Tyrosine-protein phosphatase YwqE (ywqE) CSB-YP311409BRJ
CSB-EP311409BRJ
CSB-BP311409BRJ
CSB-MP311409BRJ
CSB-EP311409BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis PGA biosynthesis protein CapA (capA) CSB-YP311410BRJ
CSB-EP311410BRJ
CSB-BP311410BRJ
CSB-MP311410BRJ
CSB-EP311410BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium pseudotuberculosis 3-dehydroquinate synthase (aroB) CSB-YP311411CQN
CSB-EP311411CQN
CSB-BP311411CQN
CSB-MP311411CQN
CSB-EP311411CQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaerhodopsin-3 (aop3), partial CSB-YP311412HUC1
CSB-EP311412HUC1
CSB-BP311412HUC1
CSB-MP311412HUC1
CSB-EP311412HUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina barkeri CoB--CoM heterodisulfide reductase iron-sulfur subunit D (hdrD) CSB-YP311413MSM
CSB-EP311413MSM
CSB-BP311413MSM
CSB-MP311413MSM
CSB-EP311413MSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0145/MT0153 (Rv0145, MT0153) CSB-YP311414MVZ
CSB-EP311414MVZ
CSB-BP311414MVZ
CSB-MP311414MVZ
CSB-EP311414MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavin-dependent monooxygenase, reductase subunit HsaB (hsaB) CSB-YP311415MVZ
CSB-EP311415MVZ
CSB-BP311415MVZ
CSB-MP311415MVZ
CSB-EP311415MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavin-dependent monooxygenase, oxygenase subunit HsaA (hsaA) CSB-YP311416MVZ
CSB-EP311416MVZ
CSB-BP311416MVZ
CSB-MP311416MVZ
CSB-EP311416MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP311417MVZ
CSB-EP311417MVZ
CSB-BP311417MVZ
CSB-MP311417MVZ
CSB-EP311417MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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