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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Uncharacterized NTE family protein ZK370.4 (ZK370.4), partial CSB-YP311601CXY
CSB-EP311601CXY
CSB-BP311601CXY
CSB-MP311601CXY
CSB-EP311601CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP311602LPG
CSB-EP311602LPG
CSB-BP311602LPG
CSB-MP311602LPG
CSB-EP311602LPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Fork head domain-containing protein FD3 (fd59A) CSB-YP311603DLU
CSB-EP311603DLU
CSB-BP311603DLU
CSB-MP311603DLU
CSB-EP311603DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 10.8 kDa protein in Gp30-rIII intergenic region (y12K) CSB-YP311604EDZ
CSB-EP311604EDZ
CSB-BP311604EDZ
CSB-MP311604EDZ
CSB-EP311604EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Protein pucC (pucC), partial CSB-YP311605RLF
CSB-EP311605RLF
CSB-BP311605RLF
CSB-MP311605RLF
CSB-EP311605RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Musca domestica Armadillo segment polarity protein (arm), partial CSB-YP311606MVC
CSB-EP311606MVC
CSB-BP311606MVC
CSB-MP311606MVC
CSB-EP311606MVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Prespore protein Dd31 (spiA), partial CSB-YP311607DKK1
CSB-EP311607DKK1
CSB-BP311607DKK1
CSB-MP311607DKK1
CSB-EP311607DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP311608HOS
CSB-EP311608HOS
CSB-BP311608HOS
CSB-MP311608HOS
CSB-EP311608HOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Pyruvate kinase (pyk), partial CSB-YP311609GFM
CSB-EP311609GFM
CSB-BP311609GFM
CSB-MP311609GFM
CSB-EP311609GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Serine--tRNA ligase (serS) CSB-YP311610SIU
CSB-EP311610SIU
CSB-BP311610SIU
CSB-MP311610SIU
CSB-EP311610SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP311611HMM
CSB-EP311611HMM
CSB-BP311611HMM
CSB-MP311611HMM
CSB-EP311611HMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein 39 (Zfp39) CSB-YP311612MO
CSB-EP311612MO
CSB-BP311612MO
CSB-MP311612MO
CSB-EP311612MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sensor protein CopS (copS), partial CSB-YP311613PWG1
CSB-EP311613PWG1
CSB-BP311613PWG1
CSB-MP311613PWG1
CSB-EP311613PWG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cold sensitive U2 snRNA suppressor 1 (CUS1) CSB-YP311614SVG
CSB-EP311614SVG
CSB-BP311614SVG
CSB-MP311614SVG
CSB-EP311614SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Probable serine/threonine-protein kinase 2 (PK2), partial CSB-YP311615EWT
CSB-EP311615EWT
CSB-BP311615EWT
CSB-MP311615EWT
CSB-EP311615EWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus UPF0102 protein Acid_2433 (Acid_2433) CSB-YP311616SIU
CSB-EP311616SIU
CSB-BP311616SIU
CSB-MP311616SIU
CSB-EP311616SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein hu-li tai shao (hts), partial CSB-YP311617DLU
CSB-EP311617DLU
CSB-BP311617DLU
CSB-MP311617DLU
CSB-EP311617DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Lon protease (lon), partial CSB-YP311618SIU
CSB-EP311618SIU
CSB-BP311618SIU
CSB-MP311618SIU
CSB-EP311618SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-like-specific protease 1 (ULP1), partial CSB-YP311619SVG
CSB-BP311619SVG
CSB-MP311619SVG
CSB-EP311619SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Increased recombination centers protein 15 (IRC15) CSB-YP311620SVG
CSB-EP311620SVG
CSB-BP311620SVG
CSB-MP311620SVG
CSB-EP311620SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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