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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe GTPase-activating protein gyp3 (gyp3), partial CSB-YP311237SXV1
CSB-EP311237SXV1
CSB-BP311237SXV1
CSB-MP311237SXV1
CSB-EP311237SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Diaminohydroxyphosphoribosylamino-pyrimidine deaminase (rib2) CSB-YP311238SXV
CSB-EP311238SXV
CSB-BP311238SXV
CSB-MP311238SXV
CSB-EP311238SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mitochondrial transcription factor 1 (MTF1) CSB-YP311239KBK
CSB-EP311239KBK
CSB-BP311239KBK
CSB-MP311239KBK
CSB-EP311239KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydrogenase A (leu2A) CSB-YP311240DOZ
CSB-EP311240DOZ
CSB-BP311240DOZ
CSB-MP311240DOZ
CSB-EP311240DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR269C (YDR269C), partial CSB-YP311241SVG1
CSB-EP311241SVG1
CSB-BP311241SVG1
CSB-MP311241SVG1
CSB-EP311241SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR327W (YDR327W) CSB-YP311242SVG
CSB-EP311242SVG
CSB-BP311242SVG
CSB-MP311242SVG
CSB-EP311242SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe RNA polymerase II transcription factor B subunit 2 (tfb2) CSB-YP311243SXV
CSB-EP311243SXV
CSB-BP311243SXV
CSB-MP311243SXV
CSB-EP311243SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mei4-dependent protein 2 (mde2) CSB-YP311244SXV
CSB-EP311244SXV
CSB-BP311244SXV
CSB-MP311244SXV
CSB-EP311244SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Green-sensitive opsin, partial CSB-YP311245OFH1
CSB-EP311245OFH1
CSB-BP311245OFH1
CSB-MP311245OFH1
CSB-EP311245OFH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelophylax ridibundus Somatostatin-2 (sst2) CSB-YP311246PEN
CSB-EP311246PEN
CSB-BP311246PEN
CSB-MP311246PEN
CSB-EP311246PEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Vitellogenin-1 (VTG1), partial CSB-YP311247CH
CSB-BP311247CH
CSB-MP311247CH
CSB-EP311247CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 2 DNA polymerase (POL), partial CSB-YP311248CCN
CSB-EP311248CCN
CSB-BP311248CCN
CSB-MP311248CCN
CSB-EP311248CCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zaire ebolavirus Envelope glycoprotein (GP), partial CSB-YP311249ZAT
CSB-BP311249ZAT
CSB-MP311249ZAT
CSB-EP311249ZAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig cytomegalovirus Envelope glycoprotein H (gH), partial CSB-YP311250GHH1
CSB-EP311250GHH1
CSB-BP311250GHH1
CSB-MP311250GHH1
CSB-EP311250GHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Tyrosine protein kinase-interacting protein, partial CSB-YP311251SAY
CSB-EP311251SAY
CSB-BP311251SAY
CSB-MP311251SAY
CSB-EP311251SAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP311252RNQ
CSB-EP311252RNQ
CSB-BP311252RNQ
CSB-MP311252RNQ
CSB-EP311252RNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tripartite terminase subunit UL28 (UL28), partial CSB-YP311253HJX
CSB-EP311253HJX
CSB-BP311253HJX
CSB-MP311253HJX
CSB-EP311253HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 DNA polymerase processivity factor (UL42) CSB-YP311254HJX
CSB-EP311254HJX
CSB-BP311254HJX
CSB-MP311254HJX
CSB-EP311254HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein UL51 (UL51) CSB-YP311255HJX
CSB-EP311255HJX
CSB-BP311255HJX
CSB-MP311255HJX
CSB-EP311255HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Probable RNA-binding protein (US11) CSB-YP311256HJX
CSB-EP311256HJX
CSB-BP311256HJX
CSB-MP311256HJX
CSB-EP311256HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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