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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Solibacter usitatus Uridylate kinase (pyrH) CSB-YP311641SIU
CSB-EP311641SIU
CSB-BP311641SIU
CSB-MP311641SIU
CSB-EP311641SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP311642SIU
CSB-EP311642SIU
CSB-BP311642SIU
CSB-MP311642SIU
CSB-EP311642SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP311643SIU1
CSB-EP311643SIU1
CSB-BP311643SIU1
CSB-MP311643SIU1
CSB-EP311643SIU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA modification GTPase MnmE (mnmE) CSB-YP311644PUC
CSB-EP311644PUC
CSB-BP311644PUC
CSB-MP311644PUC
CSB-EP311644PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP311645PUC
CSB-EP311645PUC
CSB-BP311645PUC
CSB-MP311645PUC
CSB-EP311645PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Acetylglutamate kinase (argB) CSB-YP311646PUC
CSB-EP311646PUC
CSB-BP311646PUC
CSB-MP311646PUC
CSB-EP311646PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0149 protein PA14_69010 (PA14_69010) CSB-YP311647PUC
CSB-EP311647PUC
CSB-BP311647PUC
CSB-MP311647PUC
CSB-EP311647PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable Fe (2+)-trafficking protein CSB-YP311648PUC
CSB-EP311648PUC
CSB-BP311648PUC
CSB-MP311648PUC
CSB-EP311648PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein-export protein SecB (secB) CSB-YP311649PUC
CSB-EP311649PUC
CSB-BP311649PUC
CSB-MP311649PUC
CSB-EP311649PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Sec-independent protein translocase protein TatA (tatA), partial CSB-YP311650PUC
CSB-EP311650PUC
CSB-BP311650PUC
CSB-MP311650PUC
CSB-EP311650PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L31 (rpmE) CSB-YP311651PUC
CSB-EP311651PUC
CSB-BP311651PUC
CSB-MP311651PUC
CSB-EP311651PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP311652PUC
CSB-EP311652PUC
CSB-BP311652PUC
CSB-MP311652PUC
CSB-EP311652PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S6 (rpsF) CSB-YP311653PUC
CSB-EP311653PUC
CSB-BP311653PUC
CSB-MP311653PUC
CSB-EP311653PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Urease accessory protein UreF (ureF) CSB-YP311654PUC
CSB-EP311654PUC
CSB-BP311654PUC
CSB-MP311654PUC
CSB-EP311654PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoglucosamine mutase (glmM) CSB-YP311655PUC
CSB-EP311655PUC
CSB-BP311655PUC
CSB-MP311655PUC
CSB-EP311655PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosome-binding factor A (rbfA) CSB-YP311656PUC
CSB-EP311656PUC
CSB-BP311656PUC
CSB-MP311656PUC
CSB-EP311656PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Sugar fermentation stimulation protein homolog (sfsA) CSB-YP311657PUC
CSB-EP311657PUC
CSB-BP311657PUC
CSB-MP311657PUC
CSB-EP311657PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Outer-membrane lipoprotein LolB (lolB) CSB-YP311658PUC
CSB-EP311658PUC
CSB-BP311658PUC
CSB-MP311658PUC
CSB-EP311658PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S20 (rpsT) CSB-YP311659PUC
CSB-EP311659PUC
CSB-BP311659PUC
CSB-MP311659PUC
CSB-EP311659PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP311660PUC
CSB-EP311660PUC
CSB-BP311660PUC
CSB-MP311660PUC
CSB-EP311660PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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