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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter aerogenes Histidine utilization repressor (hutC) CSB-YP320965EKN
CSB-EP320965EKN
CSB-BP320965EKN
CSB-MP320965EKN
CSB-EP320965EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein SSC1, mitochondrial (SSC1) CSB-YP320966SVG
CSB-EP320966SVG
CSB-BP320966SVG
CSB-MP320966SVG
CSB-EP320966SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Ferredoxin-like protein in nif region (fdxN) CSB-YP320967NHR
CSB-EP320967NHR
CSB-BP320967NHR
CSB-MP320967NHR
CSB-EP320967NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reovirus type 3 Microtubule-associated protein mu-2 (M1), partial CSB-YP320968RCH
CSB-EP320968RCH
CSB-BP320968RCH
CSB-MP320968RCH
CSB-EP320968RCH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein brown (bw), partial CSB-YP320969DLU1
CSB-EP320969DLU1
CSB-BP320969DLU1
CSB-MP320969DLU1
CSB-EP320969DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin B CSB-YP320970TQV
CSB-EP320970TQV
CSB-BP320970TQV
CSB-MP320970TQV
CSB-EP320970TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Polyprotein p42 (M), partial CSB-YP320971IKH
CSB-EP320971IKH
CSB-BP320971IKH
CSB-MP320971IKH
CSB-EP320971IKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Virion infectivity factor (vif) CSB-YP320972HMA
CSB-EP320972HMA
CSB-BP320972HMA
CSB-MP320972HMA
CSB-EP320972HMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpr (vpr) CSB-YP320973HMA
CSB-EP320973HMA
CSB-BP320973HMA
CSB-MP320973HMA
CSB-EP320973HMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Ribulose bisphosphate carboxylase small chain 4, chloroplastic (RBCS-4) CSB-YP320974GGV
CSB-EP320974GGV
CSB-BP320974GGV
CSB-MP320974GGV
CSB-EP320974GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia Chlorophyll a-b binding protein E, chloroplastic (CABE), partial CSB-YP320975NHC1
CSB-EP320975NHC1
CSB-BP320975NHC1
CSB-MP320975NHC1
CSB-EP320975NHC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP320976ROM
CSB-EP320976ROM
CSB-BP320976ROM
CSB-MP320976ROM
CSB-EP320976ROM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Nef (nef) CSB-YP320977SGX
CSB-EP320977SGX
CSB-BP320977SGX
CSB-MP320977SGX
CSB-EP320977SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Envelope glycoprotein gp160 (env), partial CSB-YP320978HKR
CSB-EP320978HKR
CSB-BP320978HKR
CSB-MP320978HKR
CSB-EP320978HKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag-Pol polyprotein (gag-pol), partial CSB-YP320979HKR
CSB-EP320979HKR
CSB-BP320979HKR
CSB-MP320979HKR
CSB-EP320979HKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Gag-Pol polyprotein (gag-pol), partial CSB-YP320980SGX
CSB-EP320980SGX
CSB-BP320980SGX
CSB-MP320980SGX
CSB-EP320980SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP320981HKR
CSB-EP320981HKR
CSB-BP320981HKR
CSB-MP320981HKR
CSB-EP320981HKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype A Protein Tat CSB-YP320982HKH
CSB-EP320982HKH
CSB-BP320982HKH
CSB-MP320982HKH
CSB-EP320982HKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Vpr (vpr) CSB-YP320983SGX
CSB-EP320983SGX
CSB-BP320983SGX
CSB-MP320983SGX
CSB-EP320983SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin alpha chain (PY01155) CSB-YP320984PMT
CSB-EP320984PMT
CSB-BP320984PMT
CSB-MP320984PMT
CSB-EP320984PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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