Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Uptake hydrogenase large subunit (hoxG), partial CSB-YP322056DPX
CSB-EP322056DPX
CSB-BP322056DPX
CSB-MP322056DPX
CSB-EP322056DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Early transcription factor 70 kDa subunit (VETFS), partial CSB-YP322057FEY
CSB-EP322057FEY
CSB-BP322057FEY
CSB-MP322057FEY
CSB-EP322057FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Protein FPV063 (FPV063) CSB-YP322058FEY
CSB-EP322058FEY
CSB-BP322058FEY
CSB-MP322058FEY
CSB-EP322058FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Putative CC-type chemokine FPV061 (FPV061) CSB-YP322059FEY
CSB-EP322059FEY
CSB-BP322059FEY
CSB-MP322059FEY
CSB-EP322059FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus downei Cell surface antigen I/II (spaA), partial CSB-YP322060SMJ
CSB-BP322060SMJ
CSB-MP322060SMJ
CSB-EP322060SMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar filament outer layer protein (flaA) CSB-YP322061SJS
CSB-EP322061SJS
CSB-BP322061SJS
CSB-MP322061SJS
CSB-EP322061SJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar filament 33 kDa core protein CSB-YP322062SJS
CSB-EP322062SJS
CSB-BP322062SJS
CSB-MP322062SJS
CSB-EP322062SJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar filament 34 kDa core protein CSB-YP322063THS
CSB-EP322063THS
CSB-BP322063THS
CSB-MP322063THS
CSB-EP322063THS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Flagellar filament 33 kDa core protein (flaB2) CSB-YP322064TPR
CSB-EP322064TPR
CSB-BP322064TPR
CSB-MP322064TPR
CSB-EP322064TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Volvox carteri Sulfated surface glycoprotein 185 CSB-YP322065VDT
CSB-EP322065VDT
CSB-BP322065VDT
CSB-MP322065VDT
CSB-EP322065VDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 78 kDa glucose-regulated protein homolog (GRP78), partial CSB-YP322066KBK
CSB-EP322066KBK
CSB-BP322066KBK
CSB-MP322066KBK
CSB-EP322066KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Mating-type locus allele B2 protein CSB-YP322067UBB
CSB-EP322067UBB
CSB-BP322067UBB
CSB-MP322067UBB
CSB-EP322067UBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Mating-type locus allele B5 protein CSB-YP322068UBB
CSB-EP322068UBB
CSB-BP322068UBB
CSB-MP322068UBB
CSB-EP322068UBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Regulatory protein wetA (wetA), partial CSB-YP322069EKF
CSB-EP322069EKF
CSB-BP322069EKF
CSB-MP322069EKF
CSB-EP322069EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararaca Snaclec botrocetin subunit beta CSB-YP322070BUW
CSB-EP322070BUW
CSB-BP322070BUW
CSB-MP322070BUW
CSB-EP322070BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthocidaris crassispina D-galactoside-specific lectin CSB-YP322071BZZ
CSB-EP322071BZZ
CSB-BP322071BZZ
CSB-MP322071BZZ
CSB-EP322071BZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP322072SSQ
CSB-EP322072SSQ
CSB-BP322072SSQ
CSB-MP322072SSQ
CSB-EP322072SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium tRNA (uracil (54)-C (5))-methyltransferase CSB-YP322073SXB
CSB-EP322073SXB
CSB-BP322073SXB
CSB-MP322073SXB
CSB-EP322073SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rinderpest virus Matrix protein (M) CSB-YP322075REW
CSB-EP322075REW
CSB-BP322075REW
CSB-MP322075REW
CSB-EP322075REW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prospect Hill virus Nucleoprotein (N) CSB-YP322076PSM
CSB-EP322076PSM
CSB-BP322076PSM
CSB-MP322076PSM
CSB-EP322076PSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>