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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Spinacia oleracea Fructose-bisphosphate aldolase, chloroplastic CSB-YP322260FKI
CSB-EP322260FKI
CSB-BP322260FKI
CSB-MP322260FKI
CSB-EP322260FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Chalcone synthase (CHS) CSB-YP322261PGL
CSB-EP322261PGL
CSB-BP322261PGL
CSB-MP322261PGL
CSB-EP322261PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetabularia acetabulum Ribulose bisphosphate carboxylase small chain 2, chloroplastic (RBCS-2) CSB-YP322262AAV
CSB-EP322262AAV
CSB-BP322262AAV
CSB-MP322262AAV
CSB-EP322262AAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus polyphyllus Peroxidase CSB-YP322263LKJ
CSB-EP322263LKJ
CSB-BP322263LKJ
CSB-MP322263LKJ
CSB-EP322263LKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein utxA (utxA) CSB-YP322264DUN
CSB-EP322264DUN
CSB-BP322264DUN
CSB-MP322264DUN
CSB-EP322264DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 30S ribosomal protein S17, chloroplastic (RPS17) CSB-YP322265DOA
CSB-EP322265DOA
CSB-BP322265DOA
CSB-MP322265DOA
CSB-EP322265DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class I histocompatibility antigen, A-33 alpha chain (HLA-A), partial CSB-YP322266HU
CSB-EP322266HU
CSB-BP322266HU
CSB-MP322266HU
CSB-EP322266HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Neuraminidase (NA), partial CSB-YP322267IJU
CSB-EP322267IJU
CSB-BP322267IJU
CSB-MP322267IJU
CSB-EP322267IJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Patr class I histocompatibility antigen, CH28 alpha chain, partial CSB-YP322268EQV
CSB-EP322268EQV
CSB-BP322268EQV
CSB-MP322268EQV
CSB-EP322268EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lamellibrachia sp. Giant hemoglobin linker AV-1 chain CSB-YP322269LBX
CSB-EP322269LBX
CSB-BP322269LBX
CSB-MP322269LBX
CSB-EP322269LBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catostomus commersonii Vasotocin-neurophysin VT 2 CSB-YP322270CEV
CSB-EP322270CEV
CSB-BP322270CEV
CSB-MP322270CEV
CSB-EP322270CEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster POU domain protein CF1A (vvl) CSB-YP322271DLU
CSB-EP322271DLU
CSB-BP322271DLU
CSB-MP322271DLU
CSB-EP322271DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Uncharacterized protein SCO2049 (SCO2049) CSB-YP322272FOB
CSB-EP322272FOB
CSB-BP322272FOB
CSB-MP322272FOB
CSB-EP322272FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Egg peptide speract receptor, partial CSB-YP322273FOT
CSB-EP322273FOT
CSB-BP322273FOT
CSB-MP322273FOT
CSB-EP322273FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris PI-type proteinase (prtP), partial CSB-YP322274LNF
CSB-EP322274LNF
CSB-BP322274LNF
CSB-MP322274LNF
CSB-EP322274LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Matrix protein (M) CSB-YP322275RAJ
CSB-EP322275RAJ
CSB-BP322275RAJ
CSB-MP322275RAJ
CSB-EP322275RAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dermatophagoides microceras Peptidase 1 (DERM1) CSB-YP322276DCP
CSB-EP322276DCP
CSB-BP322276DCP
CSB-MP322276DCP
CSB-EP322276DCP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar basal-body rod protein flgF (flgF) CSB-YP322277SXB
CSB-EP322277SXB
CSB-BP322277SXB
CSB-MP322277SXB
CSB-EP322277SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus sp. Wound-responsive protein GWIN3 (GWIN3) CSB-YP322278PPU
CSB-EP322278PPU
CSB-BP322278PPU
CSB-MP322278PPU
CSB-EP322278PPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Philenoptera violacea Bowman-Birk type proteinase inhibitor DE-4 CSB-YP322279PHM
CSB-EP322279PHM
CSB-BP322279PHM
CSB-MP322279PHM
CSB-EP322279PHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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