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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Proline--tRNA ligase (proS) CSB-YP322870ENV
CSB-EP322870ENV
CSB-BP322870ENV
CSB-MP322870ENV
CSB-EP322870ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase Sau3AI (sau3AIM) CSB-YP322871FKZ
CSB-EP322871FKZ
CSB-BP322871FKZ
CSB-MP322871FKZ
CSB-EP322871FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP322872ENV
CSB-EP322872ENV
CSB-BP322872ENV
CSB-MP322872ENV
CSB-EP322872ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein phnH (phnH) CSB-YP322873ENV
CSB-EP322873ENV
CSB-BP322873ENV
CSB-MP322873ENV
CSB-EP322873ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Mannan endo-1,4-beta-mannosidase A and B CSB-YP322874BBV
CSB-EP322874BBV
CSB-BP322874BBV
CSB-MP322874BBV
CSB-EP322874BBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Virion membrane protein A16 (VACWR136), partial CSB-YP322875VAI1
CSB-EP322875VAI1
CSB-BP322875VAI1
CSB-MP322875VAI1
CSB-EP322875VAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL1 (UL1), partial CSB-YP322876HWV1
CSB-EP322876HWV1
CSB-BP322876HWV1
CSB-MP322876HWV1
CSB-EP322876HWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus 65 kDa early nonstructural protein (UL84), partial CSB-YP322877HWV
CSB-EP322877HWV
CSB-BP322877HWV
CSB-MP322877HWV
CSB-EP322877HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Protein UL103 (UL103) CSB-YP322878HWV
CSB-EP322878HWV
CSB-BP322878HWV
CSB-MP322878HWV
CSB-EP322878HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL124 (UL124), partial CSB-YP322879HWV1
CSB-EP322879HWV1
CSB-BP322879HWV1
CSB-MP322879HWV1
CSB-EP322879HWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL67 (UL67) CSB-YP322880HWV
CSB-EP322880HWV
CSB-BP322880HWV
CSB-MP322880HWV
CSB-EP322880HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL13 (UL13) CSB-YP322881HWV
CSB-EP322881HWV
CSB-BP322881HWV
CSB-MP322881HWV
CSB-EP322881HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Protein UL35 (UL35), partial CSB-YP322882HWV
CSB-EP322882HWV
CSB-BP322882HWV
CSB-MP322882HWV
CSB-EP322882HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Protein UL5 (UL5) CSB-YP322883HWV
CSB-EP322883HWV
CSB-BP322883HWV
CSB-MP322883HWV
CSB-EP322883HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Capsid assembly protein UL47 (UL47), partial CSB-YP322884HWV
CSB-EP322884HWV
CSB-BP322884HWV
CSB-MP322884HWV
CSB-EP322884HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Tripartite terminase subunit UL33 homolog (UL51) CSB-YP322885HWV
CSB-EP322885HWV
CSB-BP322885HWV
CSB-MP322885HWV
CSB-EP322885HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Virion-packaging protein UL93 (UL93), partial CSB-YP322886HWV
CSB-EP322886HWV
CSB-BP322886HWV
CSB-MP322886HWV
CSB-EP322886HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Viral Fc-gamma receptor-like protein IR11, partial CSB-YP322887HWV
CSB-EP322887HWV
CSB-BP322887HWV
CSB-MP322887HWV
CSB-EP322887HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL60 (UL60) CSB-YP322888HWV
CSB-EP322888HWV
CSB-BP322888HWV
CSB-MP322888HWV
CSB-EP322888HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL125 (UL125) CSB-YP322890HWV
CSB-EP322890HWV
CSB-BP322890HWV
CSB-MP322890HWV
CSB-EP322890HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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