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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila melanogaster Protein Z600 (Z600) CSB-YP322166DLU
CSB-EP322166DLU
CSB-BP322166DLU
CSB-MP322166DLU
CSB-EP322166DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Protein P7, partial CSB-YP322167RMI
CSB-EP322167RMI
CSB-BP322167RMI
CSB-MP322167RMI
CSB-EP322167RMI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pseudofirmus ATP synthase gamma chain (atpG) CSB-YP322168BRB
CSB-EP322168BRB
CSB-BP322168BRB
CSB-MP322168BRB
CSB-EP322168BRB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Triplex capsid protein VP19C (ICP32) CSB-YP322169HJW
CSB-EP322169HJW
CSB-BP322169HJW
CSB-MP322169HJW
CSB-EP322169HJW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious pancreatic necrosis virus Structural polyprotein, partial CSB-YP322170IAU
CSB-EP322170IAU
CSB-BP322170IAU
CSB-MP322170IAU
CSB-EP322170IAU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Endoglucanase CSB-YP322171EUN
CSB-EP322171EUN
CSB-BP322171EUN
CSB-MP322171EUN
CSB-EP322171EUN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomycopsis fibuligera Beta-glucosidase 1 (BGL1), partial CSB-YP322172SAK
CSB-EP322172SAK
CSB-BP322172SAK
CSB-MP322172SAK
CSB-EP322172SAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S4E (rps4e) CSB-YP322173HTJ
CSB-EP322173HTJ
CSB-BP322173HTJ
CSB-MP322173HTJ
CSB-EP322173HTJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-activating enzyme E1 1 (UBA1), partial CSB-YP322174SVG
CSB-EP322174SVG
CSB-BP322174SVG
CSB-MP322174SVG
CSB-EP322174SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin-V immunity protein (cvi) CSB-YP322175ENL
CSB-EP322175ENL
CSB-BP322175ENL
CSB-MP322175ENL
CSB-EP322175ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African cassava mosaic virus Uncharacterized 13.1 kDa protein CSB-YP322176ADW
CSB-EP322176ADW
CSB-BP322176ADW
CSB-MP322176ADW
CSB-EP322176ADW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize streak virus genotype A Replication-associated protein A (C1) CSB-YP322177MBK
CSB-EP322177MBK
CSB-BP322177MBK
CSB-MP322177MBK
CSB-EP322177MBK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant BK polyomavirus Major capsid protein VP1 CSB-YP322178BGZ
CSB-EP322178BGZ
CSB-BP322178BGZ
CSB-MP322178BGZ
CSB-EP322178BGZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Suberization-associated anionic peroxidase 2 (TAP2) CSB-YP322179SZY
CSB-EP322179SZY
CSB-BP322179SZY
CSB-MP322179SZY
CSB-EP322179SZY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum 30S ribosomal protein S19P (rps19p) CSB-YP322180HTM
CSB-EP322180HTM
CSB-BP322180HTM
CSB-MP322180HTM
CSB-EP322180HTM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Exodeoxyribonuclease 8 (recE), partial CSB-YP322181ENV
CSB-EP322181ENV
CSB-BP322181ENV
CSB-MP322181ENV
CSB-EP322181ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (entA) CSB-YP322182ENV
CSB-EP322182ENV
CSB-BP322182ENV
CSB-MP322182ENV
CSB-EP322182ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Terminal DNA protecting protein (2) CSB-YP322183EDZ
CSB-EP322183EDZ
CSB-BP322183EDZ
CSB-MP322183EDZ
CSB-EP322183EDZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Narcissus mosaic virus RNA replication protein, partial CSB-YP322184NAR
CSB-EP322184NAR
CSB-BP322184NAR
CSB-MP322184NAR
CSB-EP322184NAR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Metallothionein-2 (MT-II) CSB-YP322185CZI
CSB-EP322185CZI
CSB-BP322185CZI
CSB-MP322185CZI
CSB-EP322185CZI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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