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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zea mays Phytochrome A (PHYA1), partial CSB-YP322570ZAX
CSB-EP322570ZAX
CSB-BP322570ZAX
CSB-MP322570ZAX
CSB-EP322570ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Tryptophan synthase beta chain (trpB) CSB-YP322571GFM
CSB-EP322571GFM
CSB-BP322571GFM
CSB-MP322571GFM
CSB-EP322571GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein 60, mitochondrial (HSP60), partial CSB-BP322572SVG
CSB-MP322572SVG
CSB-EP322572SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-2 restriction enzyme BanI (banIR) CSB-YP322573BAF
CSB-EP322573BAF
CSB-BP322573BAF
CSB-MP322573BAF
CSB-EP322573BAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus 45 kDa immediate-early protein 2 (UL122), partial CSB-YP322574HWV
CSB-EP322574HWV
CSB-BP322574HWV
CSB-MP322574HWV
CSB-EP322574HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage M2 DNA terminal protein (3) CSB-YP322575BAY
CSB-EP322575BAY
CSB-BP322575BAY
CSB-MP322575BAY
CSB-EP322575BAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage fr Lysis protein CSB-YP322576ECK
CSB-EP322576ECK
CSB-BP322576ECK
CSB-MP322576ECK
CSB-EP322576ECK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Immunoglobulin G-binding protein G (spg), partial CSB-YP322577SND
CSB-EP322577SND
CSB-BP322577SND
CSB-MP322577SND
CSB-EP322577SND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbon monoxide dehydrogenase medium chain (cutM) CSB-YP322578PUE
CSB-EP322578PUE
CSB-BP322578PUE
CSB-MP322578PUE
CSB-EP322578PUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Phosphoribulokinase, chromosomal (cfxP) CSB-YP322579DZQ
CSB-EP322579DZQ
CSB-BP322579DZQ
CSB-MP322579DZQ
CSB-EP322579DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP322580SYN
CSB-EP322580SYN
CSB-BP322580SYN
CSB-MP322580SYN
CSB-EP322580SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L30 (rpmD) CSB-YP322581BRJ
CSB-EP322581BRJ
CSB-BP322581BRJ
CSB-MP322581BRJ
CSB-EP322581BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 54S ribosomal protein L44, mitochondrial (MRPL44) CSB-YP322582SVG
CSB-EP322582SVG
CSB-BP322582SVG
CSB-MP322582SVG
CSB-EP322582SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aipysurus laevis Short neurotoxin D CSB-YP322583AFL
CSB-EP322583AFL
CSB-BP322583AFL
CSB-MP322583AFL
CSB-EP322583AFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhyparobia maderae Leucokinin-5 CSB-YP322584REJ
CSB-EP322584REJ
CSB-BP322584REJ
CSB-MP322584REJ
CSB-EP322584REJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP322585MVN
CSB-EP322585MVN
CSB-BP322585MVN
CSB-MP322585MVN
CSB-EP322585MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeotic protein distal-less (Dll) CSB-YP322586DLU
CSB-EP322586DLU
CSB-BP322586DLU
CSB-MP322586DLU
CSB-EP322586DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis Isocitrate lyase (ICL1), partial CSB-YP322587CZN
CSB-EP322587CZN
CSB-BP322587CZN
CSB-MP322587CZN
CSB-EP322587CZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Dihydroorotase (URA4) CSB-YP322588SVG
CSB-EP322588SVG
CSB-BP322588SVG
CSB-MP322588SVG
CSB-EP322588SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arachis hypogaea Putative stilbene synthase 2 CSB-YP322589ANE
CSB-EP322589ANE
CSB-BP322589ANE
CSB-MP322589ANE
CSB-EP322589ANE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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