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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Protein KRE1 (KRE1) CSB-YP322347SVG
CSB-EP322347SVG
CSB-BP322347SVG
CSB-MP322347SVG
CSB-EP322347SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Uncharacterized protein R00380 (R00380) CSB-YP322348RKU
CSB-EP322348RKU
CSB-BP322348RKU
CSB-MP322348RKU
CSB-EP322348RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Rev (rev) CSB-YP322349SGW
CSB-EP322349SGW
CSB-BP322349SGW
CSB-MP322349SGW
CSB-EP322349SGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Metapyrocatechase 2 (mcpII) CSB-YP322350CUS
CSB-EP322350CUS
CSB-BP322350CUS
CSB-MP322350CUS
CSB-EP322350CUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 9.4 kDa protein in Gp31-cd intergenic region (y13C) CSB-YP322351EDZ
CSB-EP322351EDZ
CSB-BP322351EDZ
CSB-MP322351EDZ
CSB-EP322351EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Alpha-amylase/trypsin inhibitor CM3 CSB-BP322352TQN
CSB-MP322352TQN
CSB-EP322352TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Glycine betaine/L-proline transport ATP-binding protein ProV (proV) CSB-YP322353SXB
CSB-EP322353SXB
CSB-BP322353SXB
CSB-MP322353SXB
CSB-EP322353SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morganella morganii Urease subunit gamma (ureA) CSB-YP322354MII
CSB-EP322354MII
CSB-BP322354MII
CSB-MP322354MII
CSB-EP322354MII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Megabalanus rosa Lectin BRA-2 CSB-YP322355MDB
CSB-EP322355MDB
CSB-BP322355MDB
CSB-MP322355MDB
CSB-EP322355MDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized protein 18 (VACWR018) CSB-YP322356VAI
CSB-EP322356VAI
CSB-BP322356VAI
CSB-MP322356VAI
CSB-EP322356VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C5 (C5L) CSB-YP322357VAI
CSB-EP322357VAI
CSB-BP322357VAI
CSB-MP322357VAI
CSB-EP322357VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reovirus type 2 RNA-directed RNA polymerase lambda-3 (L1), partial CSB-YP322358RCG
CSB-EP322358RCG
CSB-BP322358RCG
CSB-MP322358RCG
CSB-EP322358RCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E6 (E6) CSB-YP322359HND
CSB-EP322359HND
CSB-BP322359HND
CSB-MP322359HND
CSB-EP322359HND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype B1 subtype adw Protein P (P), partial CSB-YP322360HDV
CSB-EP322360HDV
CSB-BP322360HDV
CSB-MP322360HDV
CSB-EP322360HDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Probable minor fimbrial protein (fimZ) CSB-YP322361DDR
CSB-EP322361DDR
CSB-BP322361DDR
CSB-MP322361DDR
CSB-EP322361DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ribonucleoside-diphosphate reductase 2 subunit beta (nrdF) CSB-YP322362SXB
CSB-EP322362SXB
CSB-BP322362SXB
CSB-MP322362SXB
CSB-EP322362SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus FeMo cofactor biosynthesis protein nifB (nifB) CSB-YP322363RLC
CSB-EP322363RLC
CSB-BP322363RLC
CSB-MP322363RLC
CSB-EP322363RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyberlindnera jadinii Transaldolase-3 CSB-YP322364EPX
CSB-EP322364EPX
CSB-BP322364EPX
CSB-MP322364EPX
CSB-EP322364EPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane porin, serovar E (ompA) CSB-YP322365DSA
CSB-EP322365DSA
CSB-BP322365DSA
CSB-MP322365DSA
CSB-EP322365DSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cymbidium ringspot virus RNA-directed RNA polymerase (ORF1), partial CSB-YP322366CVN
CSB-EP322366CVN
CSB-BP322366CVN
CSB-MP322366CVN
CSB-EP322366CVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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