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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Feline immunodeficiency virus Virion infectivity factor (vif) CSB-YP322489FAM
CSB-EP322489FAM
CSB-BP322489FAM
CSB-MP322489FAM
CSB-EP322489FAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 18.1 kDa class I heat shock protein (HSP18.1) CSB-YP322490DOA
CSB-EP322490DOA
CSB-BP322490DOA
CSB-MP322490DOA
CSB-EP322490DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II reaction center protein K (psbK), partial CSB-YP322491TNI
CSB-EP322491TNI
CSB-BP322491TNI
CSB-MP322491TNI
CSB-EP322491TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp8 (8) CSB-YP322492EDZ
CSB-EP322492EDZ
CSB-BP322492EDZ
CSB-MP322492EDZ
CSB-EP322492EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nitrogenase molybdenum-iron protein beta chain (nifK1), partial CSB-YP322493RKX
CSB-EP322493RKX
CSB-BP322493RKX
CSB-MP322493RKX
CSB-EP322493RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Branched-chain amino acid transport system 2 carrier protein (braB), partial CSB-YP322494EZX
CSB-EP322494EZX
CSB-BP322494EZX
CSB-MP322494EZX
CSB-EP322494EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein phosphatase 1 regulatory subunit 1A (Ppp1r1a) CSB-YP322495RA
CSB-EP322495RA
CSB-BP322495RA
CSB-MP322495RA
CSB-EP322495RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster ATP-dependent RNA helicase p62 (Rm62), partial CSB-YP322496DLU
CSB-EP322496DLU
CSB-BP322496DLU
CSB-MP322496DLU
CSB-EP322496DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia Triosephosphate isomerase, cytosolic CSB-YP322497NHC
CSB-EP322497NHC
CSB-BP322497NHC
CSB-MP322497NHC
CSB-EP322497NHC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bean leafroll virus Major capsid protein (ORF3) CSB-YP322498BFT
CSB-EP322498BFT
CSB-BP322498BFT
CSB-MP322498BFT
CSB-EP322498BFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phanerochaete chrysosporium Manganese peroxidase H4 CSB-YP322499EUK
CSB-EP322499EUK
CSB-BP322499EUK
CSB-MP322499EUK
CSB-EP322499EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter thermosulfurogenes Xylose isomerase (xylA) CSB-YP322500TCZ
CSB-EP322500TCZ
CSB-BP322500TCZ
CSB-MP322500TCZ
CSB-EP322500TCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Inhibitory regulator protein IRA2 (IRA2), partial CSB-YP322501SVG
CSB-EP322501SVG
CSB-BP322501SVG
CSB-MP322501SVG
CSB-EP322501SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP322502NCK
CSB-EP322502NCK
CSB-BP322502NCK
CSB-MP322502NCK
CSB-EP322502NCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23, chloroplastic (rpl23) CSB-YP322503EHU
CSB-EP322503EHU
CSB-BP322503EHU
CSB-MP322503EHU
CSB-EP322503EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP322504FFZ
CSB-EP322504FFZ
CSB-BP322504FFZ
CSB-MP322504FFZ
CSB-EP322504FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 Uncharacterized protein ORF7 (ORF7) CSB-YP322505CIV
CSB-EP322505CIV
CSB-BP322505CIV
CSB-MP322505CIV
CSB-EP322505CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 Capsid protein VP1 (ORF1), partial CSB-YP322506CIV
CSB-EP322506CIV
CSB-BP322506CIV
CSB-MP322506CIV
CSB-EP322506CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens Acyl transferase (luxD) CSB-YP322507PII
CSB-EP322507PII
CSB-BP322507PII
CSB-MP322507PII
CSB-EP322507PII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physarum polycephalum Plasmin C (PLSC) CSB-YP322508EVC
CSB-EP322508EVC
CSB-BP322508EVC
CSB-MP322508EVC
CSB-EP322508EVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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