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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Undecaprenol kinase (dgkA), partial CSB-YP322549BRJ1
CSB-EP322549BRJ1
CSB-BP322549BRJ1
CSB-MP322549BRJ1
CSB-EP322549BRJ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P1 Replication protein repL (repL) CSB-YP322550EDD
CSB-EP322550EDD
CSB-BP322550EDD
CSB-MP322550EDD
CSB-EP322550EDD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lotus tetragonolobus Anti-H (O) lectin CSB-YP322551LIW
CSB-EP322551LIW
CSB-BP322551LIW
CSB-MP322551LIW
CSB-EP322551LIW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP322552ICY
CSB-EP322552ICY
CSB-BP322552ICY
CSB-MP322552ICY
CSB-EP322552ICY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP322553IGE
CSB-EP322553IGE
CSB-BP322553IGE
CSB-MP322553IGE
CSB-EP322553IGE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-1 restriction enzyme EcoEI specificity protein (hsdS) CSB-YP322554ENL
CSB-EP322554ENL
CSB-BP322554ENL
CSB-MP322554ENL
CSB-EP322554ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E6 (E6) CSB-YP322555HNQ
CSB-EP322555HNQ
CSB-BP322555HNQ
CSB-MP322555HNQ
CSB-EP322555HNQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Fusion glycoprotein F0 (F), partial CSB-YP322556MJM
CSB-BP322556MJM
CSB-MP322556MJM
CSB-EP322556MJM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaya ringspot virus Genome polyprotein CSB-YP322557PBO
CSB-EP322557PBO
CSB-BP322557PBO
CSB-MP322557PBO
CSB-EP322557PBO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Phenol hydroxylase P4 protein (dmpO) CSB-YP322558PVK
CSB-EP322558PVK
CSB-BP322558PVK
CSB-MP322558PVK
CSB-EP322558PVK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus 229E Envelope small membrane protein (E), partial CSB-YP322559HIT1
CSB-EP322559HIT1
CSB-BP322559HIT1
CSB-MP322559HIT1
CSB-EP322559HIT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 5.3 kDa protein CSB-YP322561ENL
CSB-EP322561ENL
CSB-BP322561ENL
CSB-MP322561ENL
CSB-EP322561ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS1 protein insB (insB1) CSB-YP322562SZB
CSB-EP322562SZB
CSB-BP322562SZB
CSB-MP322562SZB
CSB-EP322562SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transposase InsK for insertion sequence element IS150 (insK) CSB-YP322563ENV
CSB-EP322563ENV
CSB-BP322563ENV
CSB-MP322563ENV
CSB-EP322563ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS2 uncharacterized 16.4 kDa protein CSB-YP322564ENL
CSB-EP322564ENL
CSB-BP322564ENL
CSB-MP322564ENL
CSB-EP322564ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Dihydrodipicolinate synthase (dapA) CSB-YP322565DWY
CSB-EP322565DWY
CSB-BP322565DWY
CSB-MP322565DWY
CSB-EP322565DWY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Lipopolysaccharide 1,2-glucosyltransferase (rfaJ) CSB-YP322566SXB
CSB-EP322566SXB
CSB-BP322566SXB
CSB-MP322566SXB
CSB-EP322566SXB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA reductase (luxC) CSB-YP322567PIF
CSB-EP322567PIF
CSB-BP322567PIF
CSB-MP322567PIF
CSB-EP322567PIF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Substance P CSB-YP322568CH
CSB-EP322568CH
CSB-BP322568CH
CSB-MP322568CH
CSB-EP322568CH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus quinquestriatus Beta-insect depressant toxin LqqIT2 CSB-YP322569LDT
CSB-EP322569LDT
CSB-BP322569LDT
CSB-MP322569LDT
CSB-EP322569LDT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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