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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Glycerol-3-phosphate dehydrogenase [NAD (+)] 1 CSB-YP311439SVG
CSB-EP311439SVG
CSB-BP311439SVG
CSB-MP311439SVG
CSB-EP311439SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein kinase C-like (pkcA), partial CSB-YP311440DOZ
CSB-EP311440DOZ
CSB-BP311440DOZ
CSB-MP311440DOZ
CSB-EP311440DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Protein kinase ORF73 (ORF73), partial CSB-YP311441IAD
CSB-EP311441IAD
CSB-BP311441IAD
CSB-MP311441IAD
CSB-EP311441IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Putative membrane protein ORF6 (ORF6), partial CSB-YP311442IAD1
CSB-EP311442IAD1
CSB-BP311442IAD1
CSB-MP311442IAD1
CSB-EP311442IAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF4 (ORF4) CSB-YP311443IAD
CSB-EP311443IAD
CSB-BP311443IAD
CSB-MP311443IAD
CSB-EP311443IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF45 (ORF45) CSB-YP311444IAD
CSB-EP311444IAD
CSB-BP311444IAD
CSB-MP311444IAD
CSB-EP311444IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF33 (ORF33) CSB-YP311445IAD
CSB-EP311445IAD
CSB-BP311445IAD
CSB-MP311445IAD
CSB-EP311445IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF60 (ORF60) CSB-YP311446IAD
CSB-EP311446IAD
CSB-BP311446IAD
CSB-MP311446IAD
CSB-EP311446IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF75 (ORF75) CSB-YP311447IAD
CSB-EP311447IAD
CSB-BP311447IAD
CSB-MP311447IAD
CSB-EP311447IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Putative membrane protein ORF19 (ORF19), partial CSB-YP311448IAD1
CSB-EP311448IAD1
CSB-BP311448IAD1
CSB-MP311448IAD1
CSB-EP311448IAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF20 (ORF20) CSB-YP311449IAD
CSB-EP311449IAD
CSB-BP311449IAD
CSB-MP311449IAD
CSB-EP311449IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF26 (ORF26) CSB-YP311450IAD
CSB-EP311450IAD
CSB-BP311450IAD
CSB-MP311450IAD
CSB-EP311450IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Putative zinc-binding protein ORF11 (ORF11) CSB-YP311451IAD
CSB-EP311451IAD
CSB-BP311451IAD
CSB-MP311451IAD
CSB-EP311451IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conjugal transfer protein traG (traG), partial CSB-YP311452ENL1
CSB-EP311452ENL1
CSB-BP311452ENL1
CSB-MP311452ENL1
CSB-EP311452ENL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein disulfide-isomerase tigA (tigA) CSB-YP311453DOZ
CSB-EP311453DOZ
CSB-BP311453DOZ
CSB-MP311453DOZ
CSB-EP311453DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GTP-binding protein RHO3 (RHO3) CSB-YP311454SVG
CSB-EP311454SVG
CSB-BP311454SVG
CSB-MP311454SVG
CSB-EP311454SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Proliferating cell nuclear antigen large form CSB-YP311456DIR
CSB-EP311456DIR
CSB-BP311456DIR
CSB-MP311456DIR
CSB-EP311456DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epizootic hemorrhagic disease virus 1 Outer capsid protein VP2 (L2), partial CSB-YP311457EEW
CSB-EP311457EEW
CSB-BP311457EEW
CSB-MP311457EEW
CSB-EP311457EEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cochliobolus carbonum Exoglucanase 1 (CEL1) CSB-YP311458DUY
CSB-EP311458DUY
CSB-BP311458DUY
CSB-MP311458DUY
CSB-EP311458DUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panicum streak virus Movement protein (V2), partial CSB-YP311459PBB1
CSB-EP311459PBB1
CSB-BP311459PBB1
CSB-MP311459PBB1
CSB-EP311459PBB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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