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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Photosystem II reaction center protein I (psbI), partial CSB-YP321550FPZ
CSB-EP321550FPZ
CSB-BP321550FPZ
CSB-MP321550FPZ
CSB-EP321550FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Tat (tat) CSB-YP321551HLW
CSB-EP321551HLW
CSB-BP321551HLW
CSB-MP321551HLW
CSB-EP321551HLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Replication protein 1a (ORF1a), partial CSB-YP321552CTX
CSB-EP321552CTX
CSB-BP321552CTX
CSB-MP321552CTX
CSB-EP321552CTX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative pyrE leader peptide (pyrL) CSB-YP321553ENL
CSB-EP321553ENL
CSB-BP321553ENL
CSB-MP321553ENL
CSB-EP321553ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Elongation factor 1-alpha CSB-YP321554SZY
CSB-EP321554SZY
CSB-BP321554SZY
CSB-MP321554SZY
CSB-EP321554SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein virB4 (virB4), partial CSB-YP321555AYS
CSB-EP321555AYS
CSB-BP321555AYS
CSB-MP321555AYS
CSB-EP321555AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein virB8 (virB8), partial CSB-YP321556AYS
CSB-EP321556AYS
CSB-BP321556AYS
CSB-MP321556AYS
CSB-EP321556AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Chaperone protein DnaK (dnaK), partial CSB-YP321557DSB
CSB-EP321557DSB
CSB-BP321557DSB
CSB-MP321557DSB
CSB-EP321557DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Fimbrial assembly protein, serogroups C1 and C2 (fimB) CSB-YP321558DDR
CSB-EP321558DDR
CSB-BP321558DDR
CSB-MP321558DDR
CSB-EP321558DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Fimbrial protein (pilA), partial CSB-YP321559EZW
CSB-EP321559EZW
CSB-BP321559EZW
CSB-MP321559EZW
CSB-EP321559EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP321560SXB
CSB-EP321560SXB
CSB-BP321560SXB
CSB-MP321560SXB
CSB-EP321560SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian erythroblastosis virus Transforming protein Ski (V-SKI) CSB-YP321561ARH
CSB-EP321561ARH
CSB-BP321561ARH
CSB-MP321561ARH
CSB-EP321561ARH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar calcium-binding protein TB-17 (FCABP) CSB-YP321562TQV
CSB-EP321562TQV
CSB-BP321562TQV
CSB-MP321562TQV
CSB-EP321562TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Major capsid protein (U57), partial CSB-YP321563HJZ
CSB-EP321563HJZ
CSB-BP321563HJZ
CSB-MP321563HJZ
CSB-EP321563HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SpoIVB peptidase (spoIVB) CSB-YP321564BRJ
CSB-EP321564BRJ
CSB-BP321564BRJ
CSB-MP321564BRJ
CSB-EP321564BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Anti-sigma-B factor antagonist (rsbV) CSB-YP321565BRJ
CSB-EP321565BRJ
CSB-BP321565BRJ
CSB-MP321565BRJ
CSB-EP321565BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Broad specificity amino acid racemase CSB-YP321566FFZ
CSB-EP321566FFZ
CSB-BP321566FFZ
CSB-MP321566FFZ
CSB-EP321566FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vipera ammodytes ammodytes Phospholipase A2 homolog, ammodytin L CSB-YP321567VFO
CSB-EP321567VFO
CSB-BP321567VFO
CSB-MP321567VFO
CSB-EP321567VFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein signal peptidase (lspA), partial CSB-YP321568FFT1
CSB-EP321568FFT1
CSB-BP321568FFT1
CSB-MP321568FFT1
CSB-EP321568FFT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Aggregation substance (asa1), partial CSB-YP321569ELW
CSB-EP321569ELW
CSB-BP321569ELW
CSB-MP321569ELW
CSB-EP321569ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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